KAPA Hyper Prep Kits
Higher performance. Even from FFPE.
The KAPA Hyper Prep Kit is a versatile, streamlined solution for DNA library preparation for Illumina sequencing. The novel...
NGS Library Preparation kits
KAPA Library Preparation Kits
Innovative solutions for next-generation sequencing library preparation. Now available for Ion Torrent platforms!
KAPA Library Preparation Kits provide all of the enzymes and reaction buffers required for constructing Illumina and Ion Torrent fragment libraries and include the following modules: End Repair, A-Tailing, Ligation, and Amplification (Illumina platforms) or End Repair, Ligation and Nick Repair, and Amplification (Ion Torrent platforms). 24 barcoded adapters are also available for Ion Torrent platforms.
Benefits of the KAPA Library Preparation Kits include:
- Higher yield of adapter-ligated library molecules.
- Ultra pure, high quality library construction reagents supplied in convenient master mix formats.
- Reduced PCR bias resulting in improved sequencing coverage.
- Option of standard PCR library amplification module or a real-time PCR amplification module
- 24 barcoded adapters with <0.05% cross contamination (Ion Torrent platforms)
- Lower cost per library.
Reaction buffers are supplied in convenient, concentrated master mix formats comprising all of the required reaction components except oligonucleotide adapters or PCR primers. Similarly, a single enzyme mixture is provided for each step of the library construction process, reducing the number of pipetting steps.
The library amplification module within each KAPA Library Preparation Kit contains the KAPA HiFi HotStart DNA Polymerase, a novel enzyme engineered for high fidelity and increased affinity for DNA. The high processivity of the polymerase enables efficient amplification of targets across a broad range of GC content, minimizing bias and improving the uniformity of sequence coverage. More uniform coverage enables efficient sequencing where the highest overall coverage can be achieved from the least amount of total sequence.
KAPA Library Preparation Kits are supplied with either a standard PCR library amplification module or a real-time PCR amplification module (Illumina platforms). Real-time monitoring of library amplification provides additional information required to minimize bias due to over-amplification.
| KAPA Library Preparation Kits for Illumina Platforms | ||||
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| KK8200 | KAPA Library Preparation Kits with Standard PCR Library Amplification/Illumina series (10 libraries) |
10 libraries (10 x 50 µL reactions). Illumina series. Each kit contains End Repair Enzyme Mix (50 μL), 10X End Repair Buffer with dNTPs (100 μL), A-Tailing Enzyme (30 μL), 10X A-Tailing Buffer (50 μL), DNA Ligase (50 μL) 5X Ligation Buffer (100 μL) and 2X KAPA HiFi HotStart ReadyMix (250 μL).
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| KK8220 | KAPA Library Preparation Kits with Real-Time PCR Library Amplification/Illumina series (10 libraries) |
10 libraries (10 x 50 µL reactions). Illumina series. Each kit contains End Repair Enzyme Mix (50 μL), 10X End Repair Buffer with dNTPs (100 μL), A-Tailing Enzyme (30 μL), 10X A-Tailing Buffer (50 μL), DNA Ligase (50 μL) 5X Ligation Buffer (100 μL), 2X KAPA HiFi HotStart Real-Time PCR Master Mix (250 μL), and 4 x Fluorescent Standards (1500 μL each).
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| KK8230 | KAPA Low Throughput Library Preparation Kit with SPRI solution and Standard PCR Library Amplification/Illumina series (10 libraries) |
10 libraries (10 x 50 µL reactions). Illumina series. Each kit contains End Repair Enzyme Mix (50 μL), 10X End Repair Buffer with dNTPs (100 μL), A-Tailing Enzyme (30 μL), 10X A-Tailing Buffer (50 μL), DNA Ligase (50 μL) 5X Ligation Buffer (100 μL), 2X KAPA HiFi HotStart ReadyMix (250 μL) and PEG/NaCl SPRI solution (5 mL)
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| KK8231 | KAPA Low Throughput Library Preparation Kit with SPRI solution and no PCR Library Amplification/Illumina series (10 libraries) |
10 libraries (10 x 50 µL reactions). Illumina series. Each kit contains End Repair Enzyme Mix (50 μL), 10X End Repair Buffer with dNTPs (100 μL), A-Tailing Enzyme (30 μL), 10X A-Tailing Buffer (50 μL), DNA Ligase (50 μL), 5X Ligation Buffer (100 μL) and PEG/NaCl SPRI solution (5 mL).
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| KK8240 | KAPA Library Preparation Kits with no PCR Library Amplification/Illumina series (10 libraries) |
10 libraries (10 x 50 µL reactions). Illumina series. Each kit contains End Repair Enzyme Mix (50 μL), 10X End Repair Buffer with dNTPs (100 μL), A-Tailing Enzyme (30 μL), 10X A-Tailing Buffer (50 μL), DNA Ligase (50 μL) and 5X Ligation Buffer (100 μL).
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| KAPA Library Preparation Kits for Ion Torrent Platforms | ||||
| KK8300 | KAPA Library Preparation Kits with Standard PCR Library Amplification/Ion Torrent series (8 libraries) |
8 libraries. Ion Torrent series. Each kit contains End Repair Enzyme Mix (44 μL), 10X End Repair Buffer (62 μL), Ligation and Nick Repair Enzyme (53 µL), 5X Ligation and Nick Repair Buffer (123 µL), 10X Library Amplification Primer Mix (44 µL) and 2X KAPA HiFi HotStart ReadyMix (220 μL).
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| KK8310 | KAPA Library Preparation Kits with no Library Amplification/Ion Torrent series (8 libraries) |
8 libraries. Ion Torrent series. Each kit contains End Repair Enzyme Mix (44 μL), 10X End Repair Buffer (62 μL), Ligation and Nick Repair Enzyme (53 µL), and 5X Ligation and Nick Repair Buffer (123 µL).
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| KK8330 | KAPA non-barcoded Adapter Kits/Ion Torrent series (8 libraries) |
8 libraries. Ion Torrent series. Each kit contains 10 µM Adapter P1 (80 µL) and 10 µM Adapter A (80 µL).
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KAPA hgDNA Quantification and QC Kit Reliable quantification and quality assessment of human genomic DNA samples prior to next-generation sequencing library construction. The qua...
