KAPA Library Preparation Kits
Innovative solutions for next-generation sequencing library preparation. Now available for Ion Torrent platforms!
KAPA Library Preparation Kits provid...
NGS Library Quantification Kits
KAPA Library Quant Kits
Eliminate time-consuming and expensive titrations and reduce variability in cluster density or template-to-bead ratio with a high performance qPCR solution for next-gen sequencing library quantification.
Standard methods for quantifying next-generation sequencing libraries have a number of important disadvantages. Electrophoresis and spectrophotometry measure total nucleic acid concentrations, whereas optimal cluster density or template-to-bead ratio depend on the appropriate concentration of PCR-amplifiable DNA molecules. These methods also have low sensitivity, consuming nanograms of precious samples, and are not suitable for high-throughput workflows.
Quantitative PCR (qPCR) is inherently well-suited for next-generation sequencing library quantification:
- qPCR specifically quantifies only PCR-competent DNA molecules,
- is highly sensitive allowing accurate quantification of low concentration libraries,
- is amenable to automated liquid handling.
KAPA Library Quantification Kits are optimized for the Illumina Genome Analyzer, Roche 454 Titanium series, and Roche 454 FLX series platforms and include highly reproducible and precisely defined sets of serially diluted DNA concentration standards and state-of-the-art qPCR reagents, which contain a DNA polymerase specifically engineered for robust, SYBR® Green I-tolerant amplification of long and difficult templates.
KAPA Library Preparation Kits for higher yields of adaptor-ligated library molecules, reduced PCR-induced bias and improved sequencing coverage.
| Code | Description | Kit Contents |
|---|---|---|
| KAPA Library Quantification Kit for Illumina Sequencing Platforms | ||
| KK4824 | Library Quantification Kit - Illumina/Universal |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL), 2X KAPA SYBR FAST Universal (5 mL), and 50X ROX High/Low (200 µL each).
|
| KK4835 | Library Quantification Kit - Illumina/ABI Prism® |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL) and 2X KAPA SYBR FAST ABI Prism® (5 mL).
|
| KK4844 | Library Quantification Kit - Illumina/Bio-Rad iCycler™ |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL) and 2X KAPA SYBR® FAST Bio-Rad iCycler™ qPCR MasterMix (5 mL).
|
| KK4854 | Library Quantification Kit - Illumina/LightCycler® 480 |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL) and 2X KAPA SYBR® FAST qPCR Master Mix, optimized for Roche LightCycler® 480 (5 mL).
|
| KK4873 | Library Quantification Kit - Illumina/ROX Low |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL) and 2X KAPA SYBR FAST ROX Low (5 mL).
|
| KK4903 | Library Quantification DNA Standards only - Illumina |
DNA Standards 1 - 6 (80 µL each).
|
| KK4923 | Library Quantification Primer Premix and qPCR MasterMix only - Illumina/Universal |
10X Primer Premix (1 mL), 2X KAPA SYBR FAST Universal (5 mL) and 50X ROX High/Low (200 µL each).
|
| KK4933 | Library Quantification Primer Premix and qPCR MasterMix only - Illumina/ABI Prism® |
10X Primer Premix (1 mL) and 2X KAPA SYBR FAST ABI Prism® (5 mL).
|
| KK4943 | Library Quantification Primer Premix and qPCR MasterMix only - Illumina/Bio-Rad iCycler™ |
10X Primer Premix (1 mL) and 2X KAPA SYBR® FAST Bio-Rad iCycler™ qPCR MasterMix (5 mL).
|
| KK4953 | Library Quantification Primer Premix and qPCR MasterMix only - Illumina/LightCycler® 480 |
10X Primer Premix (1 mL) and 2X KAPA SYBR® FAST qPCR Master Mix, optimized for Roche LightCycler® 480 (5 mL).
|
| KK4973 | Library Quantification Primer Premix and qPCR MasterMix only - Illumina/ROX Low |
10X Primer Premix (1 mL) and 2X KAPA SYBR FAST ROX Low (5 mL).
|
| KAPA Library Quantification Kit for Roche 454 Sequencing Platforms | ||
| KK4820 | Library Quantification Kit - 454 FLX (Lib-A)/Universal |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL), 2X KAPA SYBR FAST Universal (5 mL), and 50X ROX High/Low (200 µL each).
|
| KK4821 | Library Quantification Kit - 454 Titanium (Lib-L)/Universal |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL), 2X KAPA SYBR FAST Universal (5 mL), and 50X ROX High/Low (200 µL each).
|
| KK4830 | Library Quantification Kit - 454 FLX (Lib-A)/ABI Prism® |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL) and 2X KAPA SYBR FAST ABI Prism® (5 mL).
|
| KK4831 | Library Quantification Kit - 454 Titanium (Lib-L)/ABI Prism® |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL) and 2X KAPA SYBR FAST ABI Prism® (5 mL).
|
| KK4840 | Library Quantification Kit - 454 FLX (Lib-A)/Bio-Rad iCycler™ |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL) and 2X KAPA SYBR® FAST Bio-Rad iCycler™ qPCR MasterMix (5 mL).
|
| KK4841 | Library Quantification Kit - 454 Titanium (Lib-L)/Bio-Rad iCycler™ |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL) and 2X KAPA SYBR® FAST Bio-Rad iCycler™ qPCR MasterMix (5 mL).
|
| KK4850 | Library Quantification Kit - 454 FLX (Lib-A)/LightCycler® 480 |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL) and 2X KAPA SYBR® FAST qPCR Master Mix, optimized for Roche LightCycler® 480 (5 mL).
|
| KK4851 | Library Quantification Kit - 454 Titanium (Lib-L)/LightCycler® 480 |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL) and 2X KAPA SYBR® FAST qPCR Master Mix, optimized for Roche LightCycler® 480 (5 mL).
|
| KK4870 | Library Quantification Kit - 454 FLX (Lib-A)/ROX Low |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL) and 2X KAPA SYBR® FAST ROX Low qPCR MasterMix (5 mL).
|
| KK4871 | Library Quantification Kit - 454 Titanium (Lib-L)/ROX Low |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL) and 2X KAPA SYBR FAST ROX Low (5 mL).
|
| KK4900 | Library Quantification DNA Standards only - 454 FLX/Lib-A |
DNA Standards 1 - 6 (80 µL each).
|
| KK4901 | Library Quantification DNA Standards only - 454 Titanium/Lib-L |
DNA Standards 1 - 6 (80 µL each).
|
| KK4920 | Library Quantification Primer Premix and qPCR MasterMix only - 454 FLX (Lib-A)/Universal |
10X Primer Premix (1 mL), 2X KAPA SYBR FAST Universal (5 mL) and 50X ROX High/Low (200 µL each).
|
| KK4921 | Library Quantification Primer Premix and qPCR MasterMix only - 454 Titanium (Lib-L)/Universal |
10X Primer Premix (1 mL), 2X KAPA SYBR® FAST Universal qPCR Master Mix (5 mL) and 50X ROX High/Low (200 µL each).
|
| KK4930 | Library Quantification Primer Premix and qPCR MasterMix only - 454 FLX (Lib-A)/ABI Prism® |
10X Primer Premix (1 mL) and 2X KAPA SYBR FAST ABI Prism® (5 mL).
|
| KK4931 | Library Quantification Primer Premix and qPCR MasterMix only - 454 Titanium (Lib-L)/ABI Prism® |
10X Primer Premix (1 mL) and 2X KAPA SYBR FAST ABI Prism® (5 mL).
|
| KK4940 | Library Quantification Primer Premix and qPCR MasterMix only - 454 FLX (Lib-A)/Bio-Rad iCycler™ |
10X Primer Premix (1 mL) and 2X KAPA SYBR® FAST Bio-Rad iCycler™ qPCR MasterMix (5 mL).
|
| KK4941 | Library Quantification Primer Premix and qPCR MasterMix only - 454 Titanium (Lib-L)/Bio-Rad iCycler™ |
10X Primer Premix (1 mL) and 2X KAPA SYBR® FAST Bio-Rad iCycler™ qPCR MasterMix (5 mL).
|
| KK4950 | Library Quantification Primer Premix and qPCR MasterMix only - 454 FLX (Lib-A)/LightCycler® 480 |
10X Primer Premix (1 mL) and 2X KAPA SYBR® FAST qPCR Master Mix, optimized for Roche LightCycler® 480 (5 mL).
|
| KK4951 | Library Quantification Primer Premix and qPCR MasterMix only - 454 Titanium (Lib-L)/LightCycler® 480 |
10X Primer Premix (1 mL) and 2X KAPA SYBR® FAST qPCR Master Mix, optimized for Roche LightCycler® 480 (5 mL).
|
| KK4970 | Library Quantification Primer Premix and qPCR MasterMix only - 454 FLX (Lib-A)/ROX Low |
10X Primer Premix (1 mL) and 2X KAPA SYBR FAST ROX Low (5 mL).
|
| KK4971 | Library Quantification Primer Premix and qPCR MasterMix only - 454 Titanium (Lib-L)/ROX Low |
10X Primer Premix (1 mL) and 2X KAPA SYBR FAST ROX Low (5 mL).
|
| KAPA Library Quantification Kit for Ion Torrent Sequencing Platforms | ||
| KK4827 | Library Quantification Kit - Ion Torrent/Universal |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL), 2X KAPA SYBR FAST Universal (5 mL), and 50X ROX High/Low (200 µL each).
|
| KK4838 | Library Quantification Kit - Ion Torrent/ABI Prism® |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL) and 2X KAPA SYBR FAST ABI Prism® (5 mL).
|
| KK4847 | Library Quantification Kit - Ion Torrent/Bio-Rad iCycler™ |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL) and 2X KAPA SYBR® FAST Bio-Rad iCycler™ qPCR MasterMix (5 mL).
|
| KK4857 | Library Quantification Kit - Ion Torrent/LightCycler® 480 |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL) and 2X KAPA SYBR® FAST qPCR Master Mix, optimized for Roche LightCycler® 480 (5 mL).
|
| KK4874 | Library Quantification Kit - Ion Torrent/ROX Low |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL) and 2X KAPA SYBR FAST ROX Low (5 mL).
|
| KK4904 | Library Quantification DNA Standards only - Ion Torrent |
DNA Standards 1 - 6 (80 µL each).
|
| KK4924 | Library Quantification Primer Premix and qPCR MasterMix only - Ion Torrent/Universal |
10X Primer Premix (1 mL), 2X KAPA SYBR FAST Universal (5 mL) and 50X ROX High/Low (200 µL each).
|
| KK4934 | Library Quantification Primer Premix and qPCR MasterMix only - Ion Torrent/ABI Prism® |
10X Primer Premix (1 mL) and 2X KAPA SYBR FAST ABI Prism® (5 mL).
|
| KK4944 | Library Quantification Primer Premix and qPCR MasterMix only - Ion Torrent/Bio-Rad iCycler™ |
10X Primer Premix (1 mL) and 2X KAPA SYBR® FAST Bio-Rad iCycler™ qPCR MasterMix (5 mL).
|
| KK4954 | Library Quantification Primer Premix and qPCR MasterMix only - Ion Torrent/LightCycler® 480 |
10X Primer Premix (1 mL) and 2X KAPA SYBR® FAST qPCR Master Mix, optimized for Roche LightCycler® 480 (5 mL).
|
| KK4974 | Library Quantification Primer Premix and qPCR MasterMix only - Ion Torrent/ROX Low |
Library Quantification Primer Premix and qPCR MasterMix only - Ion Torrent/ROX Low.
|
| KAPA Library Quantification Kit for SOLiD Sequencing Platforms | ||
| KK4823 | Library Quantification Kit - SOLiD/Universal |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL), 2X KAPA SYBR FAST Universal (5 mL), and 50X ROX High/Low (200 µL each).
|
| KK4833 | Library Quantification Kit - SOLiD/ABI Prism® |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL) and 2X KAPA SYBR FAST ABI Prism® (5 mL).
|
| KK4843 | Library Quantification Kit - SOLiD/Bio-Rad iCycler™ |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL) and 2X KAPA SYBR® FAST Bio-Rad iCycler™ qPCR MasterMix (5 mL).
|
| KK4853 | Library Quantification Kit - SOLiD/LightCycler® 480 |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL) and 2X KAPA SYBR® FAST qPCR Master Mix, optimized for Roche LightCycler® 480 (5 mL).
|
| KK4872 | Library Quantification Kit - SOLiD/ROX Low |
Complete kit containing DNA Standards 1 - 6 (80 µL each), 10X Primer Premix (1 mL) and 2X KAPA SYBR FAST ROX Low (5 mL).
|
| KK4902 | Library Quantification DNA Standards only - SOLiD |
DNA Standards 1 - 6 (80 µL each).
|
| KK4922 | Library Quantification Primer Premix and qPCR MasterMix only - SOLiD/Universal |
10X Primer Premix (1 mL), 2X KAPA SYBR FAST Universal (5 mL) and 50X ROX High/Low (200 µL each).
|
| KK4932 | Library Quantification Primer Premix and qPCR MasterMix only - SOLiD/ABI Prism® |
10X Primer Premix (1 mL) and 2X KAPA SYBR FAST ABI Prism® (5 mL).
|
| KK4942 | Library Quantification Primer Premix and qPCR MasterMix only - SOLiD/Bio-Rad iCycler™ |
10X Primer Premix (1 mL) and 2X KAPA SYBR® FAST Bio-Rad iCycler™ qPCR MasterMix (5 mL).
|
| KK4952 | Library Quantification Primer Premix and qPCR MasterMix only - SOLiD/LightCycler® 480 |
10X Primer Premix (1 mL) and 2X KAPA SYBR® FAST qPCR Master Mix, optimized for Roche LightCycler® 480 (5 mL).
|
| KK4972 | Library Quantification Primer Premix and qPCR MasterMix only - SOLiD/ROX Low |
10X Primer Premix (1 mL) and 2X KAPA SYBR FAST ROX Low (5 mL).
|
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