KAPA Library Preparation Kits
Innovative solutions for next-generation sequencing library preparation. Now available for Ion Torrent platforms!
KAPA Library Preparation Kits provid...
hgDNA Quantification and QC Kits
KAPA hgDNA Quantification and QC Kit
Reliable quantification and quality assessment of human genomic DNA samples prior to next-generation sequencing library construction.
The quantity and quality of input DNA have a profound impact on the outcome of library construction for next-generation sequencing (NGS). A number of innovations have reduced the amount of input DNA required for successful library construction, enabling routine sequencing of human DNA from a wide variety of clinically relevant sample types. Such high-throughput NGS pipelines require streamlined protocols that reduce input requirements, lower costs, improve success rates, and minimize turn-around times. A reliable method for quantifying and qualifying input DNA prior to library construction offers several benefits for any library construction workflow:
- Standardization or normalization of input DNA results in more consistent library construction outcomes (e.g. yield, library quality) with a fixed-cycle library amplification protocol, and reduces the need for sample-specific protocol adjustments.
- Quality metrics can be used to direct samples into appropriate, optimized shearing and library construction pipelines, to ensure more consistent insert sizes and data quality. Identifying the most appropriate workflow for each sample at the outset minimizes reworking of samples, and reduces average turn-around times.
The KAPA Human Genomic DNA Quantification and QC Kit is designed for the reliable quantification and quality assessment of human genomic samples including:
- Formalin-fixed paraffin-embedded (FFPE) tissue samples
- Samples obtained by laser-capture microdissection of fresh, frozen or FFPE tissue
- DNA extracted from cells collected by flow cytometry
- Free circulating DNA from plasma or serum
- Forensic samples
- Any other limiting or precious clinical sample
| Code | Description | Kit Contents |
|---|---|---|
| KK4960 | Human Genomic DNA Quantification and QC Kit - Universal |
Complete kit containing DNA Standards 1 - 5 (300 µL each), 10X 41 bp Primer Premix (200 µl), 10X 129 bp Primer Premix (200 µl), 10X 305 bp Primer Premix (200 µl), 2X KAPA SYBR® FAST Universal (3 x 1 ml), and 50X ROX High/Low (200 µl each).
|
| KK4961 | Human Genomic DNA Quantification and QC Kit - ABI Prism® |
Complete kit containing DNA Standards 1 - 5 (300 µL each), 10X 41 bp Primer Premix (200 µl), 10X 129 bp Primer Premix (200 µl), 10X 305 bp Primer Premix (200 µl), and 2X KAPA SYBR® FAST - ABI Prism® (3 x 1 ml).
|
| KK4962 | Human Genomic DNA Quantification and QC Kit - Bio-Rad iCycler™ |
Complete kit containing DNA Standards 1 - 5 (300 µL each), 10X 41 bp Primer Premix (200 µl), 10X 129 bp Primer Premix (200 µl), 10X 305 bp Primer Premix (200 µl), and 2X KAPA SYBR® FAST - Bio-Rad iCycler™ (3 x 1 ml).
|
| KK4963 | Human Genomic DNA Quantification and QC Kit optimized for Roche LightCycler® 480 |
Complete kit containing DNA Standards 1 - 5 (300 µL each), 10X 41 bp Primer Premix (200 µl), 10X 129 bp Primer Premix (200 µl), 10X 305 bp Primer Premix (200 µl), 2X KAPA SYBR® FAST optimized for LightCycler® 480 (3 x 1 ml).
|
| KK4964 | Human Genomic DNA Quantification and QC - DNA Standards only |
DNA Standards 1 - 5 (300 µl each).
|
| KK4965 | Human Genomic DNA Quantification and QC - 41 bp Primer Premix only |
10X 41 bp Primer Premix (200 µl).
|
| KK4966 | Human Genomic DNA Quantification and QC - 129 bp Primer Premix only |
10X 129 bp Primer Premix (200 µl).
|
| KK4967 | Human Genomic DNA Quantification and QC - 305 bp Primer Premix only |
10X 305 bp Primer Premix (200 µl).
|
| KK4969 | Human Genomic DNA Quantification and QC Kit - ROX Low |
Complete kit containing DNA Standards 1 - 5 (300 µL each), 10X 41 bp Primer Premix (200 µl), 10X 129 bp Primer Premix (200 µl), 10X 305 bp Primer Premix (200 µl), and 2X KAPA SYBR® FAST ROX Low (3 x 1 ml)
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