ImmunoAssay

Immunoassays

Our immunoassay technology provides unmatched analytical performance and customer value. We design assays for a wide variety of biomedical and pharmacologic research applications. In addition, our immunoassay technology eliminates many of the difficulties associated with other platforms while providing higher sensitivity and accuracy, superior result reliability, and faster, simpler workflows. MULTI‑SPOT panels multiply those benefits by quantifying multiple analytes simultaneously from a single, small-volume sample.

The majority of MSD immunoassays are sandwich assays, designed to measure the concentration of specific molecules in complex biological samples. The assays are provided in convenient 96- or 384-well microtiter plates and can be multiplexed for efficient processing. Biomolecules of interest are detected using SULFO-TAG– conjugated reporter molecules and our highly sensitive instruments. 

10 Spot Diagram

Sample Protocol for MSD Sandwich Immunoassays:
  1. Add samples and calibrators to an MSD plate coated with capture antibody, incubate, and wash away the unbound analyte.
     
  2. Add SULFO-TAG–conjugated detection antibody, incubate, and wash away unbound detection antibody.
     
  3. Add read buffer and analyze with an MSD instrument.
Comparison of U-PLEX with ELISA and Bead Based Multiplexing Technologies
FeatureU-PLEXELISABead-based
Sample Volume 25 µL 50-100 µL 50 µL
Dynamic Range 3-4 logs 1-2 logs 2-3 logs
Multiplex enabled Yes No Yes
Simple Protocol Yes No No
Number of washes
(between incubation steps)
3 4 3
Matrix effects No Yes Yes (bead aggregation)
Read Time 1 min 5 min 60 min
Instrument maintenance None Monthly calibration Clogging problems

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