nCounter Technology

The nCounter Analysis System utilizes a novel digital color-coded barcode technology that is based on direct multiplexed measurement of gene expression and offers high levels of precision and sensitivity (>1 copy per cell). The technology uses molecular "barcodes" and single molecule imaging to detect and count hundreds of unique transcripts in a single reaction.

Each color-coded barcode is attached to a single target-specific probe corresponding to a gene of interest. Mixed together with controls, they form a multiplexed CodeSet.

  • Multiplex hundreds of gene targets in a single reaction
  • High sensitivity (< 1 copy per cell)
  • Fully-automated system
  • Exceptionally easy-to-use
  • No enzymes required to perform assay
  • Multiplex 800 regions from as little as 300ng of DNA
  • 10 invariant region controls included in every CodeSet

      Molecules That Count®
Each color-coded barcode represents a single target molecule. Barcodes hybridize directly to your target molecules and can be individually counted without the need for amplification – providing very sensitive digital data.


  • NanoString’s Technology employs two ~50 base probes per mRNA that hybridize in solution. The Reporter Probe carries the signal; the Capture Probe allows the complex to be immobilized for data collection.

  • After hybridization, the excess probes are removed and the probe/target complexes aligned and immobilized in the nCounter Cartridge

  • Sample Cartridges are placed in the Digital Analyzer for data collection. Color codes on the surface of the cartridge are counted and tabulated for each target molecule.

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