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CloneSelect Imager FL

CloneSelect Imager FL, a next-generation fluorescent imaging solution for the assurance of monoclonality and automated confluence across diverse cell types

Demonstrating that cell lines are monoclonal – or that a gene was edited as expected – can be a time-consuming and highly-subjective process when relying on conventional technologies. The CloneSelect® Imager and CloneSelect® Imager FL are a high-throughput automated solutions for imaging and analyzing mammalian cells. Tracking the formation of a colony from a single cell is effortless as barcoded plates are tracked over time. Automated acquisition and analysis provides accurate, objective, and consistent results.

With high-resolution white light imaging, the CloneSelect Imager provides automated confluence, monoclonality assurance and industry leading acquisition times with the ability to image a 96-well plate in under two minutes.

The all new CloneSelect Imager FL features high contrast multichannel fluorescent and white light imaging that allows for accurate single cell detection and proof of monoclonality at day 0. Streamline your workflow with comparative confluence assays to identify and verify gene edits.

CloneSelect Imager and CloneSelect Imager FL

Verify monoclonality confidently

The all-new CloneSelect Imager FL adds high contrast multichannel fluorescent technology in addition to the standard white light imaging that allows for accurate single-cell detection and proof of monoclonality at day 0. Streamline your workflow with comparative confluence assays to identify and verify gene edits.

  • Document evidence of single cells and confluency digitally for auditing and submission to regulatory authorities
  • Image cells non invasively at multiple time points to monitor colony formation
  • Screen using high resolution white light imaging
  • Deliver real-time results with on-the-fly analysis
  • Automation and integration ready
 

Step 1: Fusion

The process of fusing B cells, expressing unique antibodies, with myeloma cells creating a hybrid cell line is called a hybridoma.

Step 2: Clone screening

The identification of clonally-derived hybridoma cell lines which are producing high amounts of monoclonal antibodies.

Step 3: Cell growth

Cell growth is determined by monitoring cell divisions over a given period of time using label-free imaging.

Step 4: Specificity and cross reactivity

The process of analyzing the ability of an antibody to bind to only one target antigen.

Step 5: Binding affinity and internalization

Binding affinity is the strength of the binding interaction between a single biomolecule (e.g. protein or DNA) to its ligand/binding partner (e.g. drug or inhibitor). Internalization is the process of monitoring the ability of a given particle to enter into the cell.

 

 

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