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Robust PCR

KAPA2G Robust PCR Kits

Streamline your PCR workflows by consolidating protocols and reaction conditions, increase pass rates, amplify difficult templates without bias, and increase sensitivity – all with a single enzyme.

The second-generation KAPA2G Robust DNA Polymerase was evolved to solve - achieve consistent amplification across a broad range of amplicon types (both GC and AT-rich). The versatility and robustness of the polymerase allows for consolidation of PCR cycling protocols and reaction conditions while increasing success rates. The high performance of the KAPA2G Robust DNA Polymerase eliminates the need for multiple enzymes and protocols - standardize your PCRs with a single enzyme solution.

The KAPA2G Robust DNA Polymerase offers:

  • Robust performance across a wide range of GC- and AT-rich templates
  • Increased PCR success rates
  • Consolidated PCR protocols and reaction conditions with a single enzyme
  • Improved tolerance to common PCR inhibitors for crude samples and/or DNA extractions
  • Consistent Colony PCR from E. coli and yeast
  • Higher yield and sensitivity per unit of enzyme
  • Three novel buffers and a proprietary PCR enhancer offer extended optimization options for the most difficult templates

KAPA2G Robust DNA Polymerase is also available with HotStart technology for improved specificity during prolonged benchtop setup or liquid handling.

CodeDescriptionKit Contents  
KAPA2G Robust DNA Polymerase
KK5004 KAPA2G Robust DNA Polymerase with dNTPs (100 U)

100 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A, 5X KAPA2G Buffer B, 5X KAPA2G GC Buffer (all with Mg2+ at a 1X conc. of 1.5 mM), 5X KAPA Enhancer 1, dNTP Mix (10 mM) and extra MgCl2 (25 mM).

 

 
KK5005 KAPA2G Robust DNA Polymerase with dNTPs (250 U)

250 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A, 5X KAPA2G Buffer B, 5X KAPA2G GC Buffer (all with Mg2+ at a 1X conc. of 1.5 mM), 5X KAPA Enhancer 1, dNTP Mix (10 mM) and extra MgCl2 (25 mM).

 

 
KK5023 KAPA2G Robust DNA Polymerase (100 U)

100 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A, 5X KAPA2G Buffer B, 5X KAPA2G GC Buffer (all with Mg2+ at a 1X conc. of 1.5 mM), 5X KAPA Enhancer 1 and extra MgCl2 (25 mM).

 

 
KK5024 KAPA2G Robust DNA Polymerase (250 U)

250 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A, 5X KAPA2G Buffer B, 5X KAPA2G GC Buffer (all with Mg2+ at a 1X conc. of 1.5 mM), 5X KAPA Enhancer 1 and extra MgCl2 (25 mM).

 

 
KAPA2G Robust HotStart DNA Polymerase
KK5515 KAPA2G Robust HotStart DNA Polymerase (250 U)

250 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A, 5X KAPA2G Buffer B, 5X KAPA2G GC Buffer (all with Mg2+ at a 1X conc. of 1.5 mM), 5X KAPA Enhancer 1 and extra MgCl2 (25 mM).

 

 
KK5516 KAPA2G Robust HotStart DNA Polymerase with dNTPs (250 U)

250 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A, 5X KAPA2G Buffer B, 5X KAPA2G GC Buffer (all with Mg2+ at a 1X conc. of 1.5 mM), 5X KAPA Enhancer 1, 10 mM dNTP Mix and extra MgCl2 (25 mM).

 

 
KK5517 KAPA2G Robust HotStart DNA Polymerase (500 U)

500 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A, 5X KAPA2G Buffer B, 5X KAPA2G GC Buffer (all with Mg2+ at a 1X conc. of 1.5 mM), 5X KAPA Enhancer 1 and extra MgCl2 (25 mM).

 

 
KK5518 KAPA2G Robust HotStart DNA Polymerase with dNTPs (500 U)

500 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A, 5X KAPA2G Buffer B, 5X KAPA2G GC Buffer (all with Mg2+ at a 1X conc. of 1.5 mM), 5X KAPA Enhancer 1, 10 mM dNTP Mix and extra MgCl2 (25 mM).

 

 
KK5522 KAPA2G Robust HotStart DNA Polymerase (100 U)

100 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A, 5X KAPA2G Buffer B, 5X KAPA2G GC Buffer (all with Mg2+ at a 1X conc. of 1.5 mM), 5X KAPA Enhancer 1 and extra MgCl2 (25 mM).

 

 
KK5532 KAPA2G Robust HotStart DNA Polymerase with dNTPs (100 U)

100 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A, 5X KAPA2G Buffer B, 5X KAPA2G GC Buffer (all with Mg2+ at a 1X conc. of 1.5 mM), 5X KAPA Enhancer 1, 10 mM dNTP Mix and extra MgCl2 (25 mM).

 

 
KK5701 KAPA2G Robust HotStart ReadyMix (100 rxn)

100 x 25 µl reactions. Convenient 2X master mix containing KAPA dNTPs, reaction buffer, and Mg2+ at a 1X final conc. of 2.0 mM. Just add template and primers.

 

 
KK5702 KAPA2G Robust HotStart ReadyMix (500 rxn)

500 x 25 µl reactions. Convenient 2X master mix containing KAPA dNTPs, reaction buffer, and Mg2+ at a 1X final conc. of 2.0 mM. Just add template and primers.

 

 
KK5704 KAPA2G Robust HotStart ReadyMix with dye (100 x 25 µL reactions)

100 x 25 µl reactions. Convenient 2X master mix containing KAPA dNTPs, KAPA2G Robust HotStart DNA Polymerase, reaction buffer, Mg2+ at a 1X final conc. of 2.0 mM, and loading dye. Just add template and primers.

 

 
KK5705 KAPA2G Robust HotStart ReadyMix with dye (500 x 25 µL reactions) 500 x 25 µl reactions. Convenient 2X master mix containing KAPA dNTPs, KAPA2G Robust HotStart DNA Polymerase, reaction buffer, Mg2+ at a 1X final conc. of 2.0 mM, and loading dye. Just add template and primers.


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