DispenCell
A simple single-cell dispenser for proof of monoclonality
The DispenCell™ Single-Cell Dispenser is an automated laboratory instrument designed for fast, easy and gentle single-cell isolation. DispenCell integrates seamlessly into your laboratory workflow, with a plug-and-play approach. Flexible and effortless, DispenCell operates equally under sterile conditions in a culture hood, or on a simple benchtop.
The DispenCell Single-Cell Dispenser utilizes advanced microfluidics technology to accurately control the dispensing of individual cells. The tool can effectively detect, size, and dispense particles down to the single-particle resolution based on impedance change that depends on particle volume. It can accommodate a wide range of cell sizes and types, allowing researchers to adjust parameters such as cell concentration and dispensing volume at a gentle pressure of less than 0.2 psi to achieve the desired cell density and distribution.
General specifications |
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Power Supply | External, PSGE120-12-01, 12v, 10A, 120W |
Plate holder | 2 plates (96 or 384 wells) |
Minimal cell number | 100 cells |
Calibration | Not required |
Cleaning | Not required |
Sample prep. | 10 min |
Cell parameters | Size, doublets |
Input voltage (External power supply) | 100 - 240 V~, 50 - 60 Hz |
Input current (External power supply) | max 2.0 A |
Input voltage (Instrument) | 12.0 V |
Max. power (Instrument) | 36 w |
Well plates compatibility | 96 WP, 200µL, (655101, Greiner) 384 WP, 50µL, (781101, Greiner) 384 WP low volume, 10µL, (788096, Greiner) |
Loading volume | per 30s load. time @2x10^⁴ cells/mL : 10µL, 200 cells |
Particle range detection capability | Dielectric, from 8µm to 25µm |
Time to dispense a 96 well plate | <= 10 min (provided Tcc < 4s) <= 13 min (provided Tcc < 6s) |
Time to dispense a 384 well plate | <= 40 min (provided Tcc < 4s) <= 52 min (provided Tcc < 6s) |
Monoclonality reliability* | >=90% |
Missed/Corrupted data points | < 1% per plate (only accounted for point serie n>6) |
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