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KAPA T4 DNA Ligase

KAPA T4 DNA Ligase catalyzes the formation of a phosphodiester bond between 5’ phosphate and 3’ hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.

KAPA T4 DNA Ligase is ideally suited for:

  • routine subcloning
  • recircularization of linear DNA
  • library construction
  • linker ligation

KAPA Rapid Ligation System

The KAPA Rapid Ligation System is a combination of KAPA Rapid T4 DNA Ligase and an optimized reaction buffer designed to ligate blunt or cohesive end DNA fragments in 5 minutes at room temperature.

 

KAPA DNA Ladders

The KAPA Express Ladder and KAPA Universal Ladder Kits are designed for determining the approximate size and quantity of double-stranded DNA on agarose gel. 

KAPA Express Ladder kits contain seven DNA fragments (in base pairs): 100, 200, 400, 800, 1600, 4000 and 8000. The KAPA Express Ladder contains one reference band (1600) for orientation.

KAPA Universal Ladder kits contain eighteen DNA fragments (in base pairs): 100, 150, 200, 300, 400, 500, 600, 800, 1000, 1200, 1600, 2000, 3000, 4000, 5000, 6000, 8000, and 10000. The KAPA Universal Ladder contains four reference bands (500, 1000, 1600, and 4000) for orientation.

All kits are formulated with DNA loading dye for direct loading on agarose gel.

Product Features:

  • Size determination of dsDNA on agarose gel
  • Formulated with loading dye for direct loading on gel
  • Stable at room temperature
  • Reference bands for orientation

 

CodeDescriptionKit Contents
KK6010 KAPA T4 DNA Ligase Kit (250 U)

5 U/µl KAPA T4 DNA Ligase (250 U) with 5x KAPA Ligation Buffer (250 µl).

 

KK6011 KAPA T4 DNA Ligase Kit (1250 U)

5 U/µl KAPA T4 DNA Ligase (1250 U) with 5x KAPA Ligation Buffer (3 x 250 µl).

 

KK6110 KAPA Rapid Ligation System (50 reactions)

25 U/µl KAPA Rapid Ligase (1250 U) with 2x KAPA Rapid Ligation Buffer (3 x 250 µl).

 

KK6111 KAPA Rapid Ligation System (150 reactions)

25 U/µl KAPA Rapid Ligase (3 x 1250 U) with 2x KAPA Rapid Ligation Buffer (7 x 250 µl).

 

CodeDescriptionKit Contents
KK6302 KAPA Universal DNA Ladder (100 ng/µL, 1 x 1 mL)

For 200 lanes, 5 µL per lane. Supplied with KAPA Loading Dye (6X) 1 x 1.5 mL.

 

KK6303 KAPA Universal DNA Ladder (100 ng/µL, 5 x 1 mL)

For 1000 lanes, 5 µL per lane. Supplied with KAPA Loading Dye (6X) 5 x 1.5 mL.

 

KK6304 KAPA Express DNA Ladder (50 ng/µL, 1 x 1 mL)

For 200 lanes, 5 µL per lane. Supplied with KAPA Loading Dye (6X) 1 x 1.5 mL.

 

KK6305 KAPA Express DNA Ladder (50 ng/µL, 5 x 1 mL)

For 1000 lanes, 5 µL per lane. Supplied with KAPA Loading Dye (6X) 5 x 1.5 mL.

 


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