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SDS & Native PAGE

SERVAGel Precast Vertical Gels

SERVAGel™ precast vertical gels for mini slab gel electrophoresis are ready-to-use, prepared from acrylamide-bis and cast into unbreakable plastic cassettes. The format of 10 x 10 cm is compatible with many slab gel tank systems. SERVAGel™ is shipped in a vacuum sealed bag.
SERVA offers precast gels in cassettes for the vertical electrophoresis of proteins. Using the new SERVAGel™ you can separate your protein samples in the presence (SDS PAGE) or absence (native PAGE) of SDS. The main benefits of the new SERVAGel™ precast gels are the separation performance, the excellent staining/destaining properties and the overall easy handling. Each gel is packed into a single bag.
With the 1 mm thin gels 10 or 12 samples can be analyzed, the separation distance is 7 cm. Poured in stable cassettes with a format of 10 x 10 x 0.7 cm the precast gels will fit perfectly into the the BlueVertical™ PRIME™ Mini Slab gel Unit. The cassette gels are compatible with most commercially available slab gel tanks, e. g. the mini vertical systems Mighty Small II (SE 260) and miniVE (SE 300) from Hoefer.

The SERVAGel™:

  • Premium resolution, superb band sharpness
  • Easy and safe to operate, no leakage
  • Short set-up times, gels are ready-to-use
  • Unbreakable plastic cassette, recyclable
  • Risk to health reduced to minimum (polymerized acrylamide, no toxic monomer)
 
SERVAGel™ Vertical Gels for 1D- and 2D-SDS PAGE available as
  • SERVAGel™ TG PRiME™ Tris/Glycine gel for standard and fast electrophoresis (approx. 35 min) according to Laemmli (Nature, 1970) with long shelf life
  • SERVAGel™ Neutral gel pH 7.4 with long shelf life, suitable for different running buffer, e.g. Tris/Glycine or Tris/Tricine, and a separation range from 5 up to 200 kDa
  • SERVAGel™ Neutral HSE gel for high speed electrophoresis (20 min) with long shelf life

The SERVAGel™ N gels (cat. nos. 43250, 43251, 43252, 43253) were developed for native gel electrophoresis. These gels can be operated in Blue and Clear Native buffer systems. Due to their neutral buffer system, the SERVAGel™ N gels feature extended shelf life.
SERVAGel™ is a trademark of SERVA.
PRiME™ stands for Premium Resolution in Minigel Electrophoresis.



SERVAGel Precast Vertical Gels


Gel Preparation & Gel Media

SERVA's ready-to-use acrylamide/bis solutions are suitable for all nucleic acid and protein electrophoresis techniques – from standard SDS-PAGE to high performance 2 D electrophoresis. Our acrylamide and N,N’-methylene bisacrylamide stock solutions allow you to cast your own gels with the desired acrylamide/bisacrylamide ratio. The solutions are made from highly purified acrylamide and N,N’-methylene bisacrylamide, stabilized and application tested. Minimize your health risk and optimize your results.

Gel Preparation & Gel Media

SDS PAGE Protein Standards

To determine the molecular weight of proteins separated in polyacrylamide gels in the presence of SDS (SDS PAGE) SERVA offers various protein markers of natural and recombinant origin. The molecular weight of the markers ranges from 5 kDa up to 245 kDa. The markers are available as ready-to-use solutions or as lyophilized protein mixtures to be dissolved in water or sample buffer.
To check the separation process during the electrophoresis run or after blotting the proteins onto a membrane you may use one of the prestained protein markers. These marker proteins are stained covalently. Due to the staining the molecular weight differs from the unstained protein and is, therefore, not suitable for molecular weight determination.

SDS PAGE Protein Standards


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