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Fast PCR

KAPA2G Fast PCR Kits

A second-generation DNA polymerase engineered for the ultimate in speed and performance.

KAPA2G Fast DNA Polymerase is a second-generation enzyme derived through a process of molecular evolution. The KAPA2G Fast DNA Polymerase was engineered for higher processivity and speed, offering significantly faster extension rates and improved PCR success rates than wild-type Taq polymerase. KAPA2G Fast is well suited for both routine and high-throughput PCR, achieving higher yields and improved sensitivity than competitor enzymes across a wide range of amplicon types.

 

KAPA2G Fast DNA Polymerase and optimized buffer system offers:

  • Extension times as low as 1 sec per kilobase.
  • Outstanding performance when compared to standard and hot start Taq polymerase.
  • 20 - 70% reductions in total PCR time.
  • No requirement for specialist PCR consumables or instrumentation.

KAPA2G Fast DNA Polymerase is also available with HotStart technology for improved specificity during prolonged benchtop setup or liquid handling.

 

 

CodeDescriptionKit Contents  
KAPA2G Fast DNA Polymerase
KK5008 KAPA2G Fast DNA Polymerase with dNTPs (100 U)

100 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM) and extra MgCl2 (25 mM).

 

 
KK5009 KAPA2G Fast DNA Polymerase with dNTPs (250 U)

250 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM) and extra MgCl2 (25 mM).

 

 
KK5020 KAPA2G Fast DNA Polymerase (100 U)

100 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM).

 

 
KK5021 KAPA2G Fast DNA Polymerase (250 U)

250 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM).

 

 
KK5101 KAPA2G Fast ReadyMix with dye (100 x 25 µL reactions)

Kit includes 100 x 25 µl reactions of KAPA2G Fast ReadyMix with dye, a convenient 2X PCR master mix containing KAPA dNTPs, KAPA2G Fast DNA Polymerase, reaction buffer, Mg2+, and loading dye.

 

 
KK5102 KAPA2G Fast ReadyMix with dye (500 x 25 µL reactions)

Kit includes 500 x 25 µl reactions of KAPA2G Fast ReadyMix with dye, a convenient 2X PCR master mix containing KAPA dNTPs, KAPA2G Fast DNA Polymerase, reaction buffer, Mg2+, and loading dye.

 

 
KAPA2G Fast HotStart DNA Polymerase
KK5500 KAPA2G Fast HotStart DNA Polymerase with dNTPs (500 U)

500 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM) and extra MgCl2 (25 mM).

 

 
KK5501 KAPA2G Fast HotStart DNA Polymerase (500 U)

500 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM).

 

 
KK5502 KAPA2G Fast HotStart DNA Polymerase with dNTPs (250 U)

250 U, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM) and extra MgCl2 (25 mM).

 

 
KK5503 KAPA2G Fast HotStart DNA Polymerase (250 U)

250 units, 5 U/µL. Supplied with proprietary 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM).

 

 
KK5510 KAPA2G Fast HotStart DNA Polymerase with dNTPs - Mg2+ free buffer (500 U)

500 units, 5 U/µL. Supplied with Mg2+ free 5X KAPA2G Buffer M, dNTP Mix (10 mM) and MgCl2 (25 mM).

 

 
KK5511 KAPA2G Fast HotStart DNA Polymerase - Mg2+ free buffer (500 U)

500 units, 5 U/µL. Supplied with Mg2+ free 5X KAPA2G Buffer M and MgCl2 (25 mM).

 

 
KK5512 KAPA2G Fast HotStart DNA Polymerase with dNTPs - Mg2+ free buffer (250 U)

250 units, 5 U/µL. Supplied with Mg2+ free 5X KAPA2G Buffer M, dNTP Mix (10 mM) and MgCl2 (25 mM).

 

 
KK5513 KAPA2G Fast HotStart DNA Polymerase - Mg2+ free buffer (250 U)

KAPA2G Fast HotStart DNA Polymerase.^(250 U, 5 U/µL). With Mg-free proprietary 5X KAPA2G Buffer M and MgCl2 (25 mM).

 

 
KK5523 KAPA2G Fast HotStart DNA Polymerase (100 U)

100 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM).

 

 
KK5530 KAPA2G Fast HotStart DNA Polymerase with dNTPs (100 U)

100 units, 5 U/µL. Supplied with 5X KAPA2G Buffer A (contains Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM) and extra MgCl2 (25 mM).

 

 
KK5601 KAPA2G Fast HotStart ReadyMix (500 x 25 µL rxns)

KAPA2G Fast HotStart DNA Polymerase in a convenient 2X ReadyMix format. Contains Mg2+ at a 1X conc. of 1.5 mM.

 

 
KK5603 KAPA2G Fast HotStart ReadyMix (100 x 25 µL rxns)

KAPA2G Fast HotStart DNA Polymerase in a convenient 2X ReadyMix format. Contains Mg2+ at a 1X conc. of 1.5 mM.

 

 
KK5608 KAPA2G Fast HotStart ReadyMix with dye (100 x 25 µL reactions)

KAPA2G Fast HotStart DNA Polymerase in a convenient 2X ReadyMix format with loading dye. Contains Mg2+ at a 1X conc. of 1.5 mM.

 

 
KK5609 KAPA2G Fast HotStart ReadyMix with dye (500 x 25 µL reactions)

KAPA2G Fast HotStart DNA Polymerase in a convenient 2X ReadyMix format with loading dye. Contains Mg2+ at a 1X conc. of 1.5 mM.

 

 

 

 


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