Isoelectric Focusing (IEF)

SERVALYT™ Carrier Ampholytes

SERVALYT™ Carrier Ampholytes are low molecular weight molecules of zwitterionic character. They are a mixture of synthetically derived species of average molecular weight distribution of 400 to 1000 dalton and comprise a multitude of varying pI-values. In agarose and polyacrylamide gels containing ampholytes, a linear pH gradient will be built up when an electric field is applied - the ampholyte molecules »carry« a net charge and thus migrate in the electric field between the electrodes as long as they will reach the position of corresponding pI. They will stop moving then and form small plateaus (stationary stacks).

Benefits are:

    • high resolution due to multimeric composition
    • fast staining and destaining times
    • clear background associated with very low unspecific binding of dyes and stains
    • high solubility in trichloroacetic acid (fast removal of ampholytes during fixation)
    • virtually no interaction with metal ions

SERVALYT™ Carrier Ampholytes

SERVALYT™ PRECOTES™ Kits are made for special applications:

  • Easy-to-start, ready-to-use

  • Large format (245 x 125 mm) for high throughput of samples in routine analysis

  • Standard format (125 x 125 mm) for screening analysis

  • Optimized particularly for the application

  • Detailed working instructions, reproducible results 


SERVAGel™ IEF 3-10

precast gel, 12 sample wells
The precast gel SERVAGel ™ IEF 3 - 10 is suitable for isoelectric focusing (IEF) in a pH range of 3 to 8.5 (Standard IEF) and 5.5 to 11 (non-equilibrium pH gradient electrophoresis, NEPHGE). For NEPHGE you change cathode and anode buffer as well as polarity of the electrophoresis chamber. In contrast to standard IEF, samples are loaded anodic, which enables an optimal separation of basic to very basic proteins.

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