Next Generation Sequencing

One system, endless possibilities

Our suite of innovative sequencing platforms – AVITI LT™, AVITI™, and AVITI24™ – empowers researchers with cutting-edge sequencing technology, flexibility, and unparalleled performance. Systems are based on our innovative avidite base chemistry and are all compatible with our library preparation solutions, including on-board library conversion, and target enrichment, and Q50 data quality, allowing each system the flexibility to upgrade as your applications and throughput needs grow.

 

DNA Sequencing

Whole-Genome Sequencing

Targeted Sequencing

Low-Pass WGS

Methylation Sequencing

16S Amplicon Sequencing

 

Whole-genome sequencing (WGS) is the most comprehensive sequencing method, determining the entire DNA sequence of a genome to identify variants. Avidite sequencing advances the accuracy and completeness of WGS in multiple ways:

  • Low GC-bias
  • Industry-leading quality specification, with > 90% of data ≥ Q30
  • Unparalleled accuracy through homopolymer regions

 

Targeted sequencing is a cost-effective method to focus sequencing resources on only the most relevant genomic regions or expressed genes. The AVITI has features that can further enhance both the time and cost efficiency of targeted sequencing methods, including:

  • Highly accurate data for robust and reliable variant calling, even at low expression levels
  • Validated ecosystem compatibility for a wide array of target capture and library prep solutions.
  • The ability to run multiple assays in parallel on two fully independent, dual flow cells
  • Low per-run costs that enable rapid sequencing of time sensitive samples, even in reduced batch sizes.
  • Customizable throughput that enables both small panels and ultra-deep sequencing on the same instrument.

Low-Pass WGS

With the steadily decreasing cost of sequencing, low pass whole genome sequencing with imputation is gaining traction as a method of genotyping. The Element AVITI™ system can help even moderate throughput labs make the leap to this high information, cost-effective method with benefits including:

  • Low per-run costs that make the many benefits of genotyping by sequencing accessible even for moderate throughput breeding programs.
  • The ability to reduce both TAT and costs by decentralizing high-volume genotyping sequencing operations with the Element $200 Genome Program
  • Seamless integration with the Gencove enterprise analytics platform for low pass imputation, simplifying genotype calling and report generation.
  • High effective coverage stemming from a low duplication rate.

Methylation Sequencing

Sequence bisulfite-converted DNA to gain insights into how chromatin remodeling impacts gene expression. With technology advances in optics, imaging algorithms, and sequencing chemistry, the AVITI offers:

  • Higher effective coverage with 5% or less spike-in needed for low diversity
  • Low GC bias
  • Industry-leading accuracy, with >90% of data Q30 or greater
  • Unparalleled accuracy through homopolymer regions

 

16S Amplicon Sequencing

16S amplicon sequencing is a popular, established sequencing method that assesses the 16S ribosomal RNA (rRNA) gene to identify and quantify the bacterial or fungal composition of a community. The Element AVITI™ System elevates the accuracy and economy of 16S amplicon sequencing:

  • Cost-effective sequencing with a low PhiX spike-in and high library plexity
  • High-accuracy results that span V1–V3, V3–V4, or full-length 16S amplicons
  • Both short read and synthetic long read options on one system

 

RNA sequencing is a powerful tool for understanding the biological state of a cell or population of cells. Gene expression data can help scientists elucidate the downstream consequences of genomic variants or understand the impact of changes to the cellular environment. The Element AVITI System lends unique strengths to RNA sequencing experiments, including:

  • Negligible index hopping, preserving the integrity of multiplexed sample data.
  • Low optical duplication rates, increasing the effective coverage of samples at a given sequencing depth.
  • Throughput conveniently matched to the sequencing requirements of typical single-cell experimental designs.
  • Isoform identification with LoopSeq long-read technology.

Single-Cell RNA Sequencing

 

Single-cell sequencing is a powerful technique for revealing hidden complexity within cell populations. Element is a validated partner for 10X Genomics, BD Rhapsody, as well as emerging instrument-free cellular barcoding technologies. With an Element AVITI™ System, you can:

 

  • Do more science for your budget with industry leading cost of $0.60 - $1 per million reads and guaranteed reagent costs for the life of your instrument.
  • Maximize cost-efficiency with throughput that neatly matches the sample outputs from both leading and emerging single-cell technologies.
  • Ensure sensitivity to low expressed genes and a high yield of genes per barcode with high quality data.
  • Enable multiple run starts daily with two fully independent 1 billion-read flow cells, providing unrivaled sequencing access in multiuser environments.
  • Use Cloudbreak Freestyle™ chemistry to load linear, third-party single-cell libraries directly on the AVITI System without manual circularization.

MicroRNA (miRNA) sequencing assesses small, noncoding RNA molecules to grant researchers insight into key biological functions for profiling and discovery applications. The Element AVITI™ System delivers consistent, accurate data at a price point that scales to serve a 2 x 75 read length and output of ~5–10 million reads per sample.

AVITI also offers the flexibility to explore both existing and novel miRNA.

  • High sequencing quality with high recovery
  • Consistent correlation and expression of miRNA
  • Ability to scale experiments for small inserts and lower output
  • Compatibility with third-party library preps via the Adept™ Workflow or Cloudbreak Freestyle™ sequencing chemistry

 

Long-read sequencing provides the additional genomic context that is often needed to reveal the full complexity of biological systems. With LoopSeq, researchers can obtain highly accurate long-read information on a short-read platform without the need for a dedicated instrument.

LoopSeq provides access to:

  • Isoform-resolved RNA sequencing data that can detect changes in gene expression that are invisible to short reads.
  • Full-length 16S data needed to identify bacteria at the species or strain level.
  • Immune repertoire data that reveal the full complexity of the immune response, including isotype or paired heavy and light chains.
  • Long-read targeted sequencing data to detect gene fusions and fully phased mutations.

 


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