DNA Sequencing Service
Bioneer Sequencing Service is a fast and highly reliable DNA Sequencing Service performed on the ABI 3730XL DNA Analyzer, which provides high quality of sequence analysis data (Phred Score (QV): ≥ 20, Guaranteed read lengths: ≥ 600bp) within 48-hour from the arrival time of sample.
Also, Bioneer’s deliberate expertise in oligo synthesis technology supports custom primer order for DNA sequencing at time.
Features and Benefits
|High-reliable Sequence Analysis:||Phred Score (QV): ≥ 20, Guaranteed read lengths: ≥ 600bp|
|Fast Turnaround Time:||48 hours from order to bench in most cases|
|Convenience - Universal primer is provided free:||Oligo Synthesis service is available together with DNA Sequencing Service|
|Competitive Pricing:||Great value for your research dollar|
Sequencing Service Workflow
Sequencing Service list
Full length sequencing (primer walking)
The Full length sequencing is a DNA sequence analysis service for whole plasmid, Viral DNA/RNA, long insert or PCR product (average more than 2kb). The turnaround time is about one (1) week on 3kb length (depending on template length). The sequence analysis result is provided sequencing data file and sequence information of designed primer in a data assembly contig file, together with primer. This service is useful for the validation of industrial strains.
The sample for gel electrophoresis process only using BigDye Terminator V3.1 on the ABI 3730XL DNA Analyzer should be sent in dry state.
Plasmid DNA mini-prep (from cultured cell)
The Plasmid DNA mini-prep is extraction of plasmid DNA from the cell cultured in a liquid medium.
PCR product clean-up (PCR purification, gel-extraction)
The PCR product clean-up is purification service required for DNA sequence analysis of PCR product. Bioneer recommends gel-extraction rather than spin-column PCR purification method.
PCR & Purification
The PCR & Purification service reaches PCR (Template and primer including PCR conditions, product size etc. should be sent), size check-up of PCR product, purification to sequencing. Also, Bioneer’s primer synthesis service is available.
Universal Primer list
|Primer name||Sequence (5' --> 3')|
|T7promoter||TAA TAC GAC TCA CTA TAG GG|
|T7terminator||GCT AGT TAT TGC TCA GCG G|
|T3||AAT TAA CCC TCA CTA AAG GG|
|SP6||ATT TAG GTG ACA CTA TAG|
|EBV-R||GTG GTT TGT CCA AAC TCA TC|
|BGH-rev||CTA GAA GGC ACA GTC GAG GC|
|M13F(-40)||GTT TTC CCA GTC ACG AC|
|M13R(-40)||CAG GAA ACA GCT ATG AC|
|M13F(-20)||GTA AAA CGA CGG CCA GT|
|M13R(-20)||GCG GAT AAC AAT TTC ACA CAG G|
|pGEX5||GGC AAG CCA CGT TTG GTG|
|pGEX3||GAG CTG CAT GTG TCA GAG G|
|pQE-forward||CCC GAA AAG TGC CAC CTG|
|pQE-reverse||GTT CTG AGG TCA TTA CTG G|
|EGFP-C||CAT GGT CCT GCT GGA GTT CGT G|
|EGFP-N||CGT CGC CGT CCA GCT CGA CCA G|
|RVprimer3||CTA GCA AAA TAG GCT GTC CC|
|RVprimer4||GAC GAT AGT CAT GCC CCG CG|
|GLprimer1||TGT ATC TTA TGG TAC TGT AAC TG|
|GLprimer2||CTT TAT GTT TTT GGC GTC TTC CA|
|CMV-F||CGC AAA TGG GCG GTA GGC GTG|
|CMV30||AAT GTC GTA ATA ACC CCG CCC CGT TGA CGC|
|CMV24||TAT TAG GAC AAG GCT GGT GGG CAC|
|Gal4AD||TAC CAC TAC AAT GGA TG|
|Gal4BD-F||TCA TCG GAA GAG AGT AG|
|Gal4BD-R||TTT CTT TGG AGC ACT TGA GC|
|MATCHMAKER3||GTG AAC TTG CGG GGT TTT TCA GTA TCT ACG AT|
|pBAD-For||ATG CCA TAG CAT TTT TAT CC|
|pBAD-Rev||GAT TTA ATC TGT ATC AGG|
|SV40-pArev||CCT CTA CAA ATG TGG TAT GG|
|SV40-pAF||AAA TAA AGC AAT AGC ATC AC|
|malEF||GGT CGT CAG ACT GTC GAT GAA GCC|
|27F||AGA GTT TGA TCM TGG CTC AG|
|1492R||TAC GGY TAC CTT GTT ACG ACT T|
|pCold-F||ACG CCA TAT CGC CGA AAG G|
|pCold-R||TCC CCG CCA AAT GGC AGG GA|