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Long Range PCR Kits

KAPA Long Range PCR Kits

Amplify long templates up to 20 kb with extreme sensitivity and increased fidelity.

The KAPA Long Range PCR system is a blend of Taq DNA polymerase and an engineered archaeal (B-family) DNA polymerase possessing proofreading capability. This two-enzyme system is designed specifically to support long range and/or sensitive PCR.

KAPA Long Range DNA Polymerase possesses 3x – 4x higher fidelity than Taq polymerase. The majority of PCR products from a KAPA Long Range HotStart DNA Polymerase reaction have a single deoxyadenosine added to the 3’ ends of the amplicon and can be used for ligation into single 3’-T -overhang cloning sites.

The KAPA Long Range PCR system is ideally suited for:

  • PCR amplification of long targets up to 20 kb and/or PCR using low amounts of template DNA.
  • Standard short- and mid-range PCR amplification.
  • Production of PCR products to be used for ligation into 3’-T-overhang cloning vectors.
  • Targeted sample enrichment for next-generation DNA sequencing.

 

CodeDescriptionKit Contents  
KAPA Long Range DNA Polymerase
KK3005 KAPA Long Range DNA Polymerase with dNTPs (250 U)

250 units, 5 U/µL. Supplied with 5X Long Range Buffer (Mg2+ free), high-quality dNTP Mix (10 mM) and MgCl2 (25 mM).

 

 
KK3006 KAPA Long Range DNA Polymerase with dNTPs (100 U)

100 units, 5 U/µL. Supplied with 5X Long Range Buffer (Mg2+ free), high-quality dNTP Mix (10 mM) and MgCl2 (25 mM).

 

 
KAPA Long Range HotStart DNA Polymerase
KK3501 KAPA Long Range HotStart DNA Polymerase with dNTPs (100 U)

100 units, 2.5 U/µL. Supplied with 5X Long Range Buffer (Mg2+ free), high-quality dNTP Mix (10 mM) and MgCl2 (25 mM).

 

 
KK3502 KAPA Long Range HotStart DNA Polymerase with dNTPs (250 U) 250 units, 2.5 U/µL. Supplied with 5X Long Range Buffer (Mg2+ free), high-quality dNTP Mix (10 mM) and MgCl2 (25 mM).  
KK3503 KAPA Long Range HotStart DNA Polymerase with dNTPs (500 U)

500 units, 2.5 U/µL. Supplied with 5X Long Range Buffer (Mg2+ free), high-quality dNTP Mix (10 mM) and MgCl2 (25 mM).

 

 
KK3601 KAPA Long Range HotStart ReadyMix with dye (100 x 25 µL reactions)

Kit includes 100 x 25 µl reactions of KAPA Long Range HotStart ReadyMix with dye, a convenient 2X PCR master mix containing KAPA dNTPs, KAPA Long Range HotStart DNA Polymerase, reaction buffer, Mg2+, and loading dye.

 

 
KK3602 KAPA Long Range HotStart ReadyMix with dye (500 x 25 µL reactions)

Kit includes 500 x 25 µl reactions of KAPA Long Range HotStart ReadyMix with dye, a convenient 2X PCR master mix containing KAPA dNTPs, KAPA Long Range HotStart DNA Polymerase, reaction buffer, Mg2+, and loading dye.

 

 

 


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