KAPA HyperPrep

KAPA Hyper Prep Kits

Higher performance. Even from FFPE.

The KAPA Hyper Prep Kit is a versatile, streamlined solution for DNA library preparation for Illumina sequencing. The novel one-tube chemistry, optimally formulated and evolved enzymes enable higher yields of adapter-ligated library and lower amplification bias. This translates to higher library diversity, lower duplication rates and more uniform coverage, particularly for FFPE and low-input samples.

• Robust performance with inputs from 1 ng – 1 µg
• Fast, one-tube workflow (2 – 3 hours) with minimal hands-on time
• Compatible with whole genome sequencing, targeted sequencing, PCR-free workflows, FFPE and low input samples
• Contains KAPA HiFi and an optimized Library Amplification Primer Mix

Figure 1: Figure 1: KAPA Hyper Prep Kits convert more input DNA to adapter-ligated library.
Libraries were prepared from Covaris-sheared DNA and quantified prior to library amplification with the KAPA Library Quantification Kit. The KAPA Hyper Prep Kits achieved the highest yield of adapter-ligated library across the range of inputs (1 µg, 100 ng or 10 ng), and required fewer cycles of amplification to generate 1 µg of amplified library required for target capture.

Figure 2: Sequencing metrics FFPE libraries prepared for whole exome sequencing.
Libraries were prepared from 100 ng of FFPE DNA, and exome capture was performed with the SeqCap EZ HGSC VCRome Design, Roche NimbleGen. Higher library yields enabled fewer cycles of amplification, leading to significantly lower duplication rates and higher target coverage.

Figure 3: The streamlined KAPA Hyper Prep workflow.
End repair, A-tailing and ligation is performed in a single tube, without intervening cleanup steps. The workflow is ideally suited for robust, high-throughput sample preparation for Illumina sequencing.

Figure 4: High performance from low input.
Key sequencing metrics for libraries prepared from 2 or 10 ng free-circulating DNA from plasma, using the KAPA Hyper Prep Kit or KAPA LTP Library Preparation Kit, with different adapter:insert molar ratios. Libraries were amplified with the Real-Time library Amplification Kit, prior to sequencing on an Illumina MiSeq. With the novel KAPA Hyper Prep chemistry, higher adapter concentrations can be used to improve library diversity.

Code	Description	Kit Contents
KK8500	KAPA Hyper Prep Kit, 8 reactions	Contains KAPA End Repair and A-tailing Enzyme Mix, End Repair and A-tailing Buffer, DNA Ligase, Ligation Buffer, KAPA HiFi HotStart ReadyMix (2X), and KAPA Library Amplification Primer Mix (10X)
KK8501	KAPA Hyper Prep Kit (without amplification module), 8 reactions	Contains KAPA End Repair and A-tailing Enzyme Mix, End Repair and A-tailing Buffer, DNA Ligase, and Ligation Buffer.
KK8502	KAPA Hyper Prep Kit, 24 reactions	Contains KAPA End Repair and A-tailing Enzyme Mix, End Repair and A-tailing Buffer, DNA Ligase, Ligation Buffer, KAPA HiFi HotStart ReadyMix (2X), and KAPA Library Amplification Primer Mix (10X)
KK8503	KAPA Hyper Prep Kit (without amplification module), 24 reactions	Contains KAPA End Repair and A-tailing Enzyme Mix, End Repair and A-tailing Buffer, DNA Ligase, and Ligation Buffer
KK8504	KAPA Hyper Prep Kit, 96 reactions	Contains KAPA End Repair and A-tailing Enzyme Mix, End Repair and A-tailing Buffer, DNA Ligase, Ligation Buffer, KAPA HiFi HotStart ReadyMix (2X), and KAPA Library Amplification Primer Mix (10X)
KK8505	KAPA Hyper Prep Kit (without amplification module), 96 reactions	Contains KAPA End Repair and A-tailing Enzyme Mix, End Repair and A-tailing Buffer, DNA Ligase, and Ligation Buffer