NGS Library Amplification Kits

KAPA Library Amplification Kits

Reduce amplification bias and improve sequencing coverage.

High fidelity PCR in NGS is used to selectively enrich library fragments carrying appropriate adaptor sequences and to amplify the amount of DNA prior to sequencing. During PCR enrichment, existing high fidelity DNA polymerases do not synthesize all library fragments with equal efficiency. This amplification bias exacerbates uneven sequence coverage.

KAPA HiFi Library Amplification Kits contain a novel DNA polymerase engineered for:

  • Improved amplification of GC- and AT-rich genomic regions
  • Reduced enzyme bias resulting in improved sequencing coverage
  • Industry leading fidelity

 

CodeDescriptionKit Contents  
KK2611 KAPA HiFi Library Amplification Kit (50 x 50 µL reactions)

50 x 50 µL reactions. Kits contain KAPA HiFi HotStart DNA Polymerase in a convenient 2X PCR master mix containing KAPA dNTPs, reaction buffer, and Mg2+ at a 1X final conc. of 2.5 mM. The kit is compatible with all Illumina NGS platforms.

 

 
KK2612 KAPA HiFi Library Amplification Kit (250 x 50 µL reactions) 250 x 50 µL reactions. Kits contain KAPA HiFi HotStart DNA Polymerase in a convenient 2X PCR master mix containing KAPA dNTPs, reaction buffer, and Mg2+ at a 1X final conc. of 2.5 mM. The kit is compatible with all Illumina NGS platforms.

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