Standard PCR

KAPA Taq DNA Polymerase is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. In the HotStart formulation, the enzyme is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step. This eliminates spurious amplification products resulting from non-specific priming events during reaction setup and initiation, and increases overall reaction efficiency and sensitivity.

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KAPA Taq and KAPA Taq HotStart DNA Polymerase is ideally suited for:

  • High throughput PCR
  • Amplification of low copy DNA templates
  • Multiplex PCR
  • Specific amplification of complex templates
  • RT-PCR

 

 

CodeDescriptionKit Contents  
KAPA Taq DNA Polymerase
KK1006

KAPA Taq ReadyMix (250 x 50 µL rxns)

KAPA Taq DNA Polymerase in a convenient 2X ReadyMix format. Contains Mg2+ at a 1X conc. of 1.5 mM.

 

 
KK1008 KAPA Taq DNA Polymerase with dNTPs (250 U)

250 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer A, 10X KAPA Taq Buffer B (both with Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM), and extra MgCl2 (25 mM).

 

 
KK1014 KAPA Taq DNA Polymerase (250 U)

250 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer A, 10X KAPA Taq Buffer B (both with Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM).

 

 
KK1015 KAPA Taq DNA Polymerase (500 U)

500 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer A, 10X KAPA Taq Buffer B (both with Mg2+ at a 1X conc. of 1.5 mM), and extra MgCl2 (25 mM).

 

 
KK1016 KAPA Taq DNA Polymerase wtih dNTPs (500 U)

500 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer A, 10X KAPA Taq Buffer B (both with Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix(10 mM) and extra MgCl2 (25 mM).

 

 
KK1020 KAPA Taq DNA Polymerase with dye (250 U)

250 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer with dye (with Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM).

 

 
KK1021 KAPA Taq DNA Polymerase with dNTPs and dye (250 U)

250 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer with dye (with Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM), and extra MgCl2 (25 mM).

 

 
KK1022 KAPA Taq DNA Polymerase with dye (500 U)

500 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer with dye (with Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM).

 

 
KK1023 KAPA Taq DNA Polymerase with dNTPs and dye (500 U)

500 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer with dye (with Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM), and extra MgCl2 (25 mM).

 

 
KK1024 KAPA Taq ReadyMix with dye (250 x 50 µL rxns)

KAPA Taq DNA Polymerase in a convenient 2X ReadyMix format. Contains Mg2+ at a 1X conc. of 1.5 mM and loading dye.

 

 
KAPA Taq HotStart DNA Polymerase
KK1508 KAPA Taq HotStart DNA Polymerase (250 U)

250 units, 5 U/µL. Wild-type Taq with HotStart antibody. Supplied with 5X KAPA Taq HotStart Buffer (Mg2+ free) and MgCl2 (25 mM).

 

 
KK1509 KAPA Taq HotStart DNA Polymerase with dNTPs (250 U)

250 U, 5 U/µL. Wild-type Taq with HotStart antibody. Supplied with 5X KAPA Taq HotStart Buffer (Mg2+ free), dNTP Mix (10 mM) and MgCl2 (25 mM).

 

 
KK1510 KAPA Taq HotStart DNA Polymerase (500 U)

500 units, 5 U/µL. Wild-type Taq with HotStart antibody. Supplied with 5X KAPA Taq HotStart Buffer (Mg2+ free) and MgCl2 (25 mM).

 

 
KK1511 KAPA Taq HotStart DNA Polymerase with dNTPs (500 U)

500 units, 5 U/µL. Wild-type Taq with HotStart antibody. Supplied with 5X KAPA Taq Buffer (Mg2+ free), dNTP Mix (10 mM) and MgCl2 (25 mM).

 

 

 

 

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