Standard PCR
KAPA Taq DNA Polymerase is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus. In the HotStart formulation, the enzyme is combined with a proprietary antibody that inactivates the enzyme until the first denaturation step. This eliminates spurious amplification products resulting from non-specific priming events during reaction setup and initiation, and increases overall reaction efficiency and sensitivity.
KAPA Taq and KAPA Taq HotStart DNA Polymerase is ideally suited for:
- High throughput PCR
- Amplification of low copy DNA templates
- Multiplex PCR
- Specific amplification of complex templates
- RT-PCR
| Code | Description | Kit Contents | ||
|---|---|---|---|---|
| KAPA Taq DNA Polymerase | ||||
| KK1006 |
KAPA Taq ReadyMix (250 x 50 µL rxns) |
KAPA Taq DNA Polymerase in a convenient 2X ReadyMix format. Contains Mg2+ at a 1X conc. of 1.5 mM.
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| KK1008 | KAPA Taq DNA Polymerase with dNTPs (250 U) |
250 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer A, 10X KAPA Taq Buffer B (both with Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM), and extra MgCl2 (25 mM).
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| KK1014 | KAPA Taq DNA Polymerase (250 U) |
250 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer A, 10X KAPA Taq Buffer B (both with Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM).
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| KK1015 | KAPA Taq DNA Polymerase (500 U) |
500 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer A, 10X KAPA Taq Buffer B (both with Mg2+ at a 1X conc. of 1.5 mM), and extra MgCl2 (25 mM).
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| KK1016 | KAPA Taq DNA Polymerase wtih dNTPs (500 U) |
500 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer A, 10X KAPA Taq Buffer B (both with Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix(10 mM) and extra MgCl2 (25 mM).
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| KK1020 | KAPA Taq DNA Polymerase with dye (250 U) |
250 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer with dye (with Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM).
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| KK1021 | KAPA Taq DNA Polymerase with dNTPs and dye (250 U) |
250 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer with dye (with Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM), and extra MgCl2 (25 mM).
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| KK1022 | KAPA Taq DNA Polymerase with dye (500 U) |
500 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer with dye (with Mg2+ at a 1X conc. of 1.5 mM) and extra MgCl2 (25 mM).
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| KK1023 | KAPA Taq DNA Polymerase with dNTPs and dye (500 U) |
500 units, 5 U/µL. Wild-type Taq with 10X KAPA Taq Buffer with dye (with Mg2+ at a 1X conc. of 1.5 mM), dNTP Mix (10 mM), and extra MgCl2 (25 mM).
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| KK1024 | KAPA Taq ReadyMix with dye (250 x 50 µL rxns) |
KAPA Taq DNA Polymerase in a convenient 2X ReadyMix format. Contains Mg2+ at a 1X conc. of 1.5 mM and loading dye.
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| KAPA Taq HotStart DNA Polymerase | ||||
| KK1508 | KAPA Taq HotStart DNA Polymerase (250 U) |
250 units, 5 U/µL. Wild-type Taq with HotStart antibody. Supplied with 5X KAPA Taq HotStart Buffer (Mg2+ free) and MgCl2 (25 mM).
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| KK1509 | KAPA Taq HotStart DNA Polymerase with dNTPs (250 U) |
250 U, 5 U/µL. Wild-type Taq with HotStart antibody. Supplied with 5X KAPA Taq HotStart Buffer (Mg2+ free), dNTP Mix (10 mM) and MgCl2 (25 mM).
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| KK1510 | KAPA Taq HotStart DNA Polymerase (500 U) |
500 units, 5 U/µL. Wild-type Taq with HotStart antibody. Supplied with 5X KAPA Taq HotStart Buffer (Mg2+ free) and MgCl2 (25 mM).
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| KK1511 | KAPA Taq HotStart DNA Polymerase with dNTPs (500 U) |
500 units, 5 U/µL. Wild-type Taq with HotStart antibody. Supplied with 5X KAPA Taq Buffer (Mg2+ free), dNTP Mix (10 mM) and MgCl2 (25 mM).
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