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The genesig® Easy Kit for African Swine Fever Virus (ASFV) is designed for the in vitro detection of ASFV genomes. The kit is designed to have a broad detection profile. Specifically, the primers will detect over 95% of sequences available on the NCBI database at the time of design or last review. 

Porcine reproductive and respiratory syndrome virus (PRRSV) is the pathogen responsible for porcine reproductive and respiratory syndrome, a highly impactful disease in the global swine industry. Infection causes reproductive failure in breeding animals, and respiratory illness in young pigs and piglets. 

PRRSV is an RNA virus classified into two major genotypes: PRRSV-1 (PRRSV-EU) and PRRSV-2 (PRRSV-US, also known as PRRSV-NA [North America]). 

PRRSV-EU, first identified in Europe, includes strains like the Lelystad virus and is further divided into subtypes, some of which are highly virulent. 

PRRSV-US is predominant in North America and Asia, and is known for its genetic diversity across multiple lineages. 

Highly Pathogenic PRRSV (PRRSV-HP) is a variant that originated from the PRRSV-US genotype. It is distinguished by a unique deletion in the genome. Infection causes severe clinical signs, including high fever, cyanosis, vomiting, and neurological symptoms, often resulting in high mortality rates among piglets. 

Specificity 

The genesig®PLEX kit for PRRSV is designed for the in vitro detection of PRRSV-US, PRRSV-EU and PRRSV-HP genotypes. Specifically, the primers will detect 95% of PRRSV-US and PRRSV-EU sequences available on the NCBI database at the time of last review. Due to genomic variation of PRRSV-HP strains and restrictions to maintain exclusivity, the primers will detect 79% of PRRSV-HP sequences. 

The PRRSV-US assay may cross-react with rat arterivirus.