คุณลักษณะโปรตีน Protein Characterization

Chemiluminescent Western Detection

Everything you need for chemiluminescent Western blotting

Advansta's WesternBright line of HRP substrates contains a solution for every chemiluminescent Western experiment, from routine blotting to quantitative comparisons, from film detection to CCD imaging. Each substrate has been optimized for high-performance enhanced chemiluminescence detection (Figure 1) for a specific experiment type, ensuring successful, reproducible Western blots for every sample and situation.


Complete kit for multicolor fluorescent Western blotting

WesternBright MCF visible and near infrared (IR) fluorescent Western blotting kits allow the assay of two proteins at once, increasing the quality and quantity of information that can be gained from a single blot. Assay a control alongside a protein of interest. Assay phosphorylated and un-phosphorylated isoforms of a protein simultaneously. The fluorescent dyes provided with WesternBright MCF and WesternBright MCF-IR outperform Cy™ dyes and allow detection of low pg of protein. The WesternBright MCF protocols save time and money since there is no need for disposable film, and the blot can be imaged immediately, without drying.

  • Fluorescent detection – of two proteins, with sensitivity 10x greater than Cy dyes
  • AdvanBlock™-PF – blocking solution for fast, protein-free blocking and greater signal-to-noise ratios
  • High sensitivity – detect picograms of protein
  • Fast results – entire protocol can be completed in 3.5 hours
  • WesternBright MCF – compatible with imaging systems that detect Cy3 and Cy5 or similar dyes
  • WesternBright MCF-IR – compatible with near-IR imaging systems
  • AdvanWash and AdvanWash-IR – washing solutions optimized for chemiluminescent and fluorescent or for near-IR fluorescent Western blots

Visitors: 149,029