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HiFi Uracil+ (bisulfite)

KAPA HiFi Uracil+

Take the fight out of bisulfite sequencing!

KAPA HiFi DNA Polymerase provides high fidelity next-generation sequencing library amplification with the highest yield, lowest bias, and most uniform sequence coverage available. These characteristics are retained in KAPA HiFi Uracil+ DNA Polymerase, a modified version of KAPA HiFi engineered to tolerate uracil residues. The new enzyme is particularly well suited for applications employing bisulfite DNA conversion, which typically produces low concentrations of AT-rich DNA.

KAPA HiFi HotStart Uracil+ ReadyMix (2X) is a ready-to-use cocktail containing all components required for PCR, except primers and template.

Applications:

  • Methylation analysis - amplification of bisulfite-converted DNA for sequencing
  • Amplification of damaged DNA samples
  • Prevention of false-positive results due to carryover amplicon contamination

 

CodeDescriptionKit Contents  
KK2801 KAPA HiFi HotStart Uracil+ ReadyMix (1 x 1.25 mL) 50 x 50 µL reactions. Convenient 2X master mix containing KAPA HiFi HotStart Uracil+ DNA Polymerase, KAPA dNTPs, reaction buffer, and Mg2+ at a 1X final conc. of 2.5 mM. Just add template and primers.  
KK2802 KAPA HiFi HotStart Uracil+ ReadyMix (1 x 6.25 mL) 250 x 50 µL reactions. Convenient 2X master mix containing KAPA HiFi HotStart Uracil+ DNA Polymerase, KAPA dNTPs, reaction buffer, and Mg2+ at a 1X final conc. of 2.5 mM. Just add template and primers.  

 

 


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