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High Fidelity PCR Kits

KAPA HiFi PCR Kits

A second-generation polymerase engineered for extreme fidelity, robustness, and uniform amplification of AT- and GC-rich templates. 100x fidelity improvement compared to Taq polymerase.

KAPA Library Preparation Kits containing the novel KAPA HiFi DNA Polymerase are recommended for NGS library construction and amplifcation. Product information can be found here>>

KAPA Library Amplification Kits containing the novel KAPA HiFi DNA Polymerase are recommended for NGS library amplification. Product information can be found here>>

KAPA HiFi DNA Polymerase is a novel, single-enzyme system that exhibits industry-leading performance when compared with other high fidelity polymerases and polymerase blends. KAPA HiFi has been engineered to have an increased affinity for DNA without the need for accessory protein domains. The intrinsic high processivity of KAPA HiFi DNA Polymerase results in significant improvements to yield, sensitivity, speed, target length, and the ability to amplify difficult templates (e.g GC-rich) - standardize your high fidelity PCRs with a single enzyme.

The engineered KAPA HiFi DNA Polymerase plus optimized buffer system offers:

  • Superior fidelity - 100x improvement compared to Taq polymerase.
  • Robust performance across a wide range of difficult templates, including both AT- and GC-rich amplicons.
  • Consolidation of PCR protocols and reaction conditions with single enzyme.
  • Long range amplification of complex targets - up to 15 kb from genomic DNA.
  • High speed - reduce reaction times by up to 75%.

KAPA HiFi DNA Polymerase is also available with HotStart technology for improved specificity during prolonged benchtop setup or liquid handling.

 

CodeDescriptionKit Contents  
KAPA HiFi DNA Polymerase
KK2101 KAPA HiFi DNA Polymerase with dNTPs (100 U)

100 U, 1 U/µL. Supplied with 5X Fidelity Buffer, 5X GC Buffer (both with Mg2+ at a 1X conc. of 2 mM), high-quality dNTP Mix (10 mM) and extra MgCl2 (25 mM).

 

 
KK2102 KAPA HiFi DNA Polymerase with dNTPs (250 U)

250 units, 1 U/µL. Supplied with 5X Fidelity Buffer, 5X GC Buffer (both with Mg2+ at a 1X conc. of 2 mM), high-quality dNTP Mix (10 mM) and extra MgCl2 (25 mM).

 

 
KAPA HiFi HotStart DNA Polymerase
KK2501 KAPA HiFi HotStart DNA Polymerase with dNTPs (100 U)

100 units, 1 U/µL. Supplied with 5X Fidelity Buffer, 5X GC Buffer (both with Mg2+ at a 1X conc. of 2 mM), high-quality dNTP Mix (10 mM) and extra MgCl2 (25 mM).

 

 
KK2502 KAPA HiFi HotStart DNA Polymerase with dNTPs (250 U)

250 units, 1 U/µL. Supplied with 5X Fidelity Buffer, 5X GC Buffer (both with Mg2+ at a 1X conc. of 2 mM), high-quality dNTP Mix (10 mM) and extra MgCl2 (25 mM).

 

 
KK2601 KAPA HiFi HotStart ReadyMix (100 rxn)

100 x 25 µl reactions. Convenient 2X master mix containing KAPA dNTPs, reaction buffer, and Mg2+ at a 1X final conc. of 2.5 mM. Just add template and primers.

 

 
KK2602 KAPA HiFi HotStart ReadyMix (500 rxn)

500 x 25 µl reactions. Convenient 2X master mix containing KAPA dNTPs, reaction buffer, and Mg2+ at a 1X final conc. of 2.5 mM. Just add template and primers.

 

 

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