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DNA Sequencing Service

Bioneer Sequencing Service is a fast and highly reliable DNA Sequencing Service performed on the ABI 3730XL DNA Analyzer, which provides high quality of sequence analysis data (Phred Score (QV): ≥ 20, Guaranteed read lengths: ≥ 600bp) within 48-hour from the arrival time of sample.
Also, Bioneer’s deliberate expertise in oligo synthesis technology supports custom primer order for DNA sequencing at time.

 

Features and Benefits

 High-reliable Sequence Analysis: Phred Score (QV): ≥ 20, Guaranteed read lengths: ≥ 600bp
 Fast Turnaround Time: 48 hours from order to bench in most cases
 Convenience - Universal primer is provided free: Oligo Synthesis service is available together with DNA Sequencing Service
 Competitive Pricing: Great value for your research dollar

Sequencing Service Workflow

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Sequencing Service list

 Standard sequencing
 Full length sequencing (primer walking)
The Full length sequencing is a DNA sequence analysis service for whole plasmid, Viral DNA/RNA, long insert or PCR product (average more than 2kb). The turnaround time is about one (1) week on 3kb length (depending on template length). The sequence analysis result is provided sequencing data file and sequence information of designed primer in a data assembly contig file, together with primer. This service is useful for the validation of industrial strains.
 ready-to-load(without editing)
The sample for gel electrophoresis process only using BigDye Terminator V3.1 on the ABI 3730XL DNA Analyzer should be sent in dry state.



Additional Service

 Plasmid DNA mini-prep (from cultured cell)
The Plasmid DNA mini-prep is extraction of plasmid DNA from the cell cultured in a liquid medium.

 PCR product clean-up (PCR purification, gel-extraction)
The PCR product clean-up is purification service required for DNA sequence analysis of PCR product. Bioneer recommends gel-extraction rather than spin-column PCR purification method.

 PCR & Purification
The PCR & Purification service reaches PCR (Template and primer including PCR conditions, product size etc. should be sent), size check-up of PCR product, purification to sequencing. Also, Bioneer’s primer synthesis service is available.



Universal Primer list

Primer name Sequence (5' --> 3')
T7promoter TAA TAC GAC TCA CTA TAG GG
T7terminator GCT AGT TAT TGC TCA GCG G
T3 AAT TAA CCC TCA CTA AAG GG
SP6 ATT TAG GTG ACA CTA TAG
EBV-R GTG GTT TGT CCA AAC TCA TC
BGH-rev CTA GAA GGC ACA GTC GAG GC
M13F(-40) GTT TTC CCA GTC ACG AC
M13R(-40) CAG GAA ACA GCT ATG AC
M13F(-20) GTA AAA CGA CGG CCA GT
M13R(-20) GCG GAT AAC AAT TTC ACA CAG G
pGEX5 GGC AAG CCA CGT TTG GTG
pGEX3 GAG CTG CAT GTG TCA GAG G
pQE-forward CCC GAA AAG TGC CAC CTG
pQE-reverse GTT CTG AGG TCA TTA CTG G
EGFP-C CAT GGT CCT GCT GGA GTT CGT G
EGFP-N CGT CGC CGT CCA GCT CGA CCA G
RVprimer3 CTA GCA AAA TAG GCT GTC CC
RVprimer4 GAC GAT AGT CAT GCC CCG CG
GLprimer1 TGT ATC TTA TGG TAC TGT AAC TG
GLprimer2 CTT TAT GTT TTT GGC GTC TTC CA
CMV-F CGC AAA TGG GCG GTA GGC GTG
CMV30 AAT GTC GTA ATA ACC CCG CCC CGT TGA CGC
CMV24 TAT TAG GAC AAG GCT GGT GGG CAC
Gal4AD TAC CAC TAC AAT GGA TG
Gal4BD-F TCA TCG GAA GAG AGT AG
Gal4BD-R TTT CTT TGG AGC ACT TGA GC
MATCHMAKER3 GTG AAC TTG CGG GGT TTT TCA GTA TCT ACG AT
pBAD-For ATG CCA TAG CAT TTT TAT CC
pBAD-Rev GAT TTA ATC TGT ATC AGG
SV40-pArev CCT CTA CAA ATG TGG TAT GG
SV40-pAF AAA TAA AGC AAT AGC ATC AC
malEF GGT CGT CAG ACT GTC GAT GAA GCC
27F AGA GTT TGA TCM TGG CTC AG
1492R TAC GGY TAC CTT GTT ACG ACT T
pCold-F ACG CCA TAT CGC CGA AAG G
pCold-R TCC CCG CCA AAT GGC AGG GA

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    Standard custom from Bioneer For routine applications, Bioneer offers standard custom oligos with no modification, Bio-RP purification, provided in a tube format. Standard oligonuc...
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