a newchromogenic DNAin situhybridization (ISH)technology. DNAscope™ employs the proven “double-Z” probe design and signal amplification system of RNAscope™, enablingrapid and flexi...
Advanced Cell Diagnostics
RNAscope® and BaseScope™ Assays Overview
The RNAscope® and BaseScope™ Assays are based on the proprietary RNAscope® Technology, enabling RNA target expression analysis within intact cells and tissues with high sensitivity and specificity.
RNAscope® Assay
The innovative RNAscope® Assay, with in situ hybridization technology to detect mRNA and ncRNA targets of >300bp, was launched in 2011 and marks the foundation of our product portfolio. The proprietary probe design requires an average of 20 Z pairs per target, however a minimum hybridization of 6 Z pairs can be used to obtain a signal. With over 800 publications in peer-reviewed journals, across multiple disciplines, the RNAscope® Assay represents a revolutionary advance in in situ target RNA detection.
BaseScope™ Assay
The BaseScope™ Assay is a recent addition to the RNAscope® Technology product portfolio, launched in early 2016. Using the same innovative technology and proprietary probe design as used in the RNAscope Assay, the BaseScope™ Assay is further refined to detect as few as 1-3 Z pairs. This powerful in situ hybridization technology enables the specific detection of exon junctions, short target/splice variants highly homologous sequences and point mutations, with single cell detection sensitivity in a broad range of tissues, samples and species.
RNAscope® and BaseScope™ Assay Comparison
| RNAscope® Assay | BaseScope™ Assay | |
|---|---|---|
| Number of ZZ Pair per target | Standard probe design is 20 ZZ probes (minimum of 6 ZZ probes) | 1 to 3 ZZ probes based on the application |
| Target | mRNA >300 bases lncRNA > 300 bases |
Exon junctions/Splice Variants RNA 50 to 300 bases Validated point mutations |
| Application | lcnRNA and mRNA >300 bases | Exon junctions/splice variants, circular RNA, gene fusion, gene knockout Short/highly homologous sequences, TCRs and CDR sequence for T cell clones, pre-miRNA, gene editing/CRISPR, CAR-T cell validation and detection Point mutation, short InDel, homologues |
| Multiplex capability | Single to up to 12-plex | Single to Duplex |
| Detection | Chromogenic or fluorescent | Chromogenic |
| Sample type | FFPE cells & tissues (TMAs) Fresh frozen tissues Fixed frozen tissues Cultured cells |
FFPE cells & tissues (TMAs) Fresh frozen tissues Fixed frozen tissues Cultured cells |
| Pretreatment kit | Reagent Kit includes Universal Pretreatment Kit | Reagent kit includes Universal Pretreatment Kit |
| Kit | RNAscope® kit* | BaseScope™ kit* |
| Probe | RNAscope® probe* | BaseScope™ probe* |
| Automation | Available on Leica Bond Rx and Ventana Discovery ULTRA | Singleplex available on Leica Bond Rx and Ventana Discovery ULTRA |
*RNAscope® and BaseScope™ Assay products components cannot be interchanged.
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