TriM Scope™ II Multiphoton Microscope
The TriM Scope™ II is a modular platform for intravital multiphoton microscopy and optogenetic manipulation, which can be equipped with a variety of lasers, scanner configurations, detectors, and dedicated modules for in vivo imaging. Thanks to its modularity, the system can be adapted to various applications, including deep tissue multicolor imaging, intravital 3D time-lapse measurements, and calcium monitoring under natural and biased conditions. Thus, the TriM Scope II Multiphoton Microscope can be used for a large variety of applications, ranging from developmental biology to immuno-oncology and neuroscience.
Scanners
Scanner options for the TriM Scope II Multiphoton Microscope
| Resonant scanner | Galvo scanner | ||
|---|---|---|---|
| Unidirectional scan | Bidirectional scan | ||
| Scan frequency | 8 kHz | 1.2 kHz | 2.0 kHz |
| Frame rate | 30 fps at 512×512 pixels | 2.3 fps at 512×512 pixels | 3.3 fps at 512×512 pixels |
| Scan field (with 20× objective) | 500 µm × 500 µm | 500 µm × 500 µm | |
Detectors
Detector options for the TriM Scope II Multiphoton Microscope
| GaAs "red" | GaAsP | Multialkali "blue" | Multialkali "green/red" | |
|---|---|---|---|---|
| Quantum efficiency at peak wavelength | 12% at 800 nm | 40% at 580 nm | 25% at 375 nm | 18% at 480 nm |
| Spectral response | 380–890 nm | 300–720 nm | 230–870 nm | 230–920 nm |
| Sensitivity adjustment range | 1:50 | 1:50 | 1:10,000 | 1:10,000 |
Camera options for the TriM Scope II Multiphoton Microscope
| sCMOS camera | CMOS camera | |
|---|---|---|
| Sensor type | Front-illuminated scientific CMOS | Front-illuminated CMOS |
| Sensor format | 4.2 megapixels | 4.2 megapixels |
| Active pixels (w × h) | 2048×2048 | 2048×2048 |
| Pixel size | 6.5 µm × 6.5 µm | 5.5 µm × 5.5 µm |
| Sensor size | 13.3 mm × 13.3 mm, 18.8 mm diagonal | 11.26 mm x 11.26 mm , 15.9 mm diagonal |
| Pixel well depth | 45,000 e- | 13,500 e- |
| Pixel frame rate | 40 fps (100 fps optional) | 80 fps |
| Readout mode | Rolling shutter | Global shutter |
| Read noise [median] rolling shutter | 2.1 e- | 13e- |
| Interface | USB 3.1 (Camera Link optional) | USB 3.0 |
| Quantum efficiency | 80% | >50% |
Lasers
Laser options for the TriM Scope II Multiphoton Microscope
| Wavelength (nm) | Models (examples) | |
|---|---|---|
| Ultra-wide tunable | 680–960, 960–1300, 1025 | CRONUS laser |
| 680–1300 | Spectra Physics InSight® X3+, Coherent® Chameleon Discovery NX TPC | |
| Ti:sapphire | 690–1080 | Coherent® Chameleon Ultra II, Spectra Physics MaiTai® |
| OPO | 1010–1340 | Coherent® Chameleon MPX (pumped with Ultra II) |
| OP(CP)A | 1300–1700 | Class 5 Photonics White Dwarf, Light Conversion® I-OPA, Spectra Physics® Spirit-NOPA®-VISIR |
| Fixed-wavelength femtosecond | 920, 1040, 1064 | Coherent® Axon Series, Spark Lasers® Alcor Series |
| Visible | 405, 445, 488, 514, 532, 552 (others on request) | Coherent® OBIS™ |
