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Total RNA Isolation

A unique feature of the STRATEC Molecular RNA Kits is the selective removal of genomic DNA from the lysate before RNA purification This step eliminates the hassle and cost of DNase digestion, which is generally required with kits from other suppliers to avoid interference of DNA in RT-PCR reactions.

Advantages:

  • high-quality total RNA from different starting materials
  • DNA removal without expensive DNase digestion
  • time saving and convenient handling
  • high, reproducible yields
  • accurate, reliable gene expression data
  • versatile use - parallel RNA and protein isolation, RNA and DNA isolation, RNA CleanUp from Trizol phases

Kit Overview

Sample Source Recommended Kit Sample Volume Yield
Human and animal cells InviTrap Spin Cell RNA Mini Kit
InviTrap Spin Universal RNA Mini Kit
up to 107cells up to 150 µg
Human and animal tissue InviTrap Spin Tissue RNA Mini Kit
InviTrap Spin Universal RNA Mini Kit
up to 20 mg
up to 20 mg
up to 100 µg
Bacteria and yeast InviTrap Spin Cell RNA Mini Kit
up to 109 bacterial cells, up to 5 x 107 yeast cells depending on type and amount of starting material
Whole blood InviTrap Spin Blood RNA Mini Kit
InviTrap Spin Universal RNA Mini Kit
up to 1.5 ml up to 1 - 5 µg total RNA / ml of whole blood
Serum and plasma Invisorb Spin Virus RNA Mini Kit up to 200 µl depending on viral titer
Plant cells and tissue, filamentous fungi InviTrap Spin Plant RNA Mini Kit 10 – 100 mg fresh plant tissue,
up to 107 plant or fungal cells
up to 100 µg

 

Workflow

The InviTrap RNA kits combine an efficient, stringent lysis and an inactivation of endogenous and exogenous RNases with the selective removal of genomic DNA by binding at the surface of the mineral carrier particles provided within the provided Lysis Solutions and simultaneously to the lysis of the starting material. Selective binding of the DNA is assured by optimized buffer conditions and the mineral carrier particles loaded with the genomic DNA are removed by spin down or by centrifugation on the spin filter surface. The RNA remains in the supernatant in the filtrate and is - after the adjustment of special binding conditions- bound onto the membrane of the RNA-RTA Spin Filter. Contaminants are removed by repeated washing steps and the purified total RNA which is almost free of DNA contaminations can be eluted in a small volume of RNase free water.

Products which are CE-marked according to the IVD-Directive (Directive 98/79/EC on in vitro diagnostic medical devices) can be used for diagnostic applications in countries where this directive is recognized.

ProductPackage SizeCatalogue No
InviTrap Spin Universal RNA Mini Kit 50 purifications 1060100200
(old: 10603002)
InviTrap Spin Universal RNA Mini Kit 250 purifications 1060100300
(old: 10603003)

 

InviTrap Spin Cell RNA Mini Kit 50 purifications 1061100200
(old: 10605002)
InviTrap Spin Cell RNA Mini Kit 250 purifications 1061100300
(old: 10605003)

 

InviTrap Spin Tissue RNA Mini Kit 50 purifications 1062100200
(old: 10607002)
InviTrap Spin Tissue RNA Mini Kit 250 purifications 1062100300
(old: 10607003)

 

InviTrap Spin Plant RNA Mini Kit 50 purifications 1064100300
InviTrap Spin Plant RNA Mini Kit 250 purifications 1064100400

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