HiFi Uracil+ (bisulfite)
KAPA HiFi Uracil+
Take the fight out of bisulfite sequencing!
KAPA HiFi DNA Polymerase provides high fidelity next-generation sequencing library amplification with the highest yield, lowest bias, and most uniform sequence coverage available. These characteristics are retained in KAPA HiFi Uracil+ DNA Polymerase, a modified version of KAPA HiFi engineered to tolerate uracil residues. The new enzyme is particularly well suited for applications employing bisulfite DNA conversion, which typically produces low concentrations of AT-rich DNA.
KAPA HiFi HotStart Uracil+ ReadyMix (2X) is a ready-to-use cocktail containing all components required for PCR, except primers and template.
Applications:
- Methylation analysis - amplification of bisulfite-converted DNA for sequencing
- Amplification of damaged DNA samples
- Prevention of false-positive results due to carryover amplicon contamination
| Code | Description | Kit Contents | ||
|---|---|---|---|---|
| KK2801 | KAPA HiFi HotStart Uracil+ ReadyMix (1 x 1.25 mL) | 50 x 50 µL reactions. Convenient 2X master mix containing KAPA HiFi HotStart Uracil+ DNA Polymerase, KAPA dNTPs, reaction buffer, and Mg2+ at a 1X final conc. of 2.5 mM. Just add template and primers. | ||
| KK2802 | KAPA HiFi HotStart Uracil+ ReadyMix (1 x 6.25 mL) | 250 x 50 µL reactions. Convenient 2X master mix containing KAPA HiFi HotStart Uracil+ DNA Polymerase, KAPA dNTPs, reaction buffer, and Mg2+ at a 1X final conc. of 2.5 mM. Just add template and primers. | ||
