SERVA DNA Stain G is a safer alternative to traditional ethidium bromide stain for detecting nucleic acid in agarose gels. It is at least as sensitive as ethidium bromide and can b...
SDS & Native PAGE
SERVAGel Precast Vertical Gels
SERVAGel™ precast vertical gels for mini slab gel electrophoresis are ready-to-use, prepared from acrylamide-bis and cast into unbreakable plastic cassettes. The format of 10 x 10 cm is compatible with many slab gel tank systems. SERVAGel™ is shipped in a vacuum sealed bag.
SERVA offers precast gels in cassettes for the vertical electrophoresis of proteins. Using the new SERVAGel™ you can separate your protein samples in the presence (SDS PAGE) or absence (native PAGE) of SDS. The main benefits of the new SERVAGel™ precast gels are the separation performance, the excellent staining/destaining properties and the overall easy handling. Each gel is packed into a single bag.
With the 1 mm thin gels 10 or 12 samples can be analyzed, the separation distance is 7 cm. Poured in stable cassettes with a format of 10 x 10 x 0.7 cm the precast gels will fit perfectly into the the BlueVertical™ PRIME™ Mini Slab gel Unit. The cassette gels are compatible with most commercially available slab gel tanks, e. g. the mini vertical systems Mighty Small II (SE 260) and miniVE (SE 300) from Hoefer.
The SERVAGel™:
- Premium resolution, superb band sharpness
- Easy and safe to operate, no leakage
- Short set-up times, gels are ready-to-use
- Unbreakable plastic cassette, recyclable
- Risk to health reduced to minimum (polymerized acrylamide, no toxic monomer)
SERVAGel™ Vertical Gels for 1D- and 2D-SDS PAGE available as
The SERVAGel™ N gels (cat. nos. 43250, 43251, 43252, 43253) were developed for native gel electrophoresis. These gels can be operated in Blue and Clear Native buffer systems. Due to their neutral buffer system, the SERVAGel™ N gels feature extended shelf life.
SERVAGel™ is a trademark of SERVA.
PRiME™ stands for Premium Resolution in Minigel Electrophoresis.
- SERVAGel™ TG PRiME™ Tris/Glycine gel for standard and fast electrophoresis (approx. 35 min) according to Laemmli (Nature, 1970) with long shelf life
- SERVAGel™ Neutral gel pH 7.4 with long shelf life, suitable for different running buffer, e.g. Tris/Glycine or Tris/Tricine, and a separation range from 5 up to 200 kDa
- SERVAGel™ Neutral HSE gel for high speed electrophoresis (20 min) with long shelf life
The SERVAGel™ N gels (cat. nos. 43250, 43251, 43252, 43253) were developed for native gel electrophoresis. These gels can be operated in Blue and Clear Native buffer systems. Due to their neutral buffer system, the SERVAGel™ N gels feature extended shelf life.
SERVAGel™ is a trademark of SERVA.
PRiME™ stands for Premium Resolution in Minigel Electrophoresis.
SERVAGel Precast Vertical Gels
Gel Preparation & Gel Media
SERVA's ready-to-use acrylamide/bis solutions are suitable for all nucleic acid and protein electrophoresis techniques – from standard SDS-PAGE to high performance 2 D electrophoresis. Our acrylamide and N,N’-methylene bisacrylamide stock solutions allow you to cast your own gels with the desired acrylamide/bisacrylamide ratio. The solutions are made from highly purified acrylamide and N,N’-methylene bisacrylamide, stabilized and application tested. Minimize your health risk and optimize your results.
Gel Preparation & Gel Media
SDS PAGE Protein Standards
To determine the molecular weight of proteins separated in polyacrylamide gels in the presence of SDS (SDS PAGE) SERVA offers various protein markers of natural and recombinant origin. The molecular weight of the markers ranges from 5 kDa up to 245 kDa. The markers are available as ready-to-use solutions or as lyophilized protein mixtures to be dissolved in water or sample buffer.
To check the separation process during the electrophoresis run or after blotting the proteins onto a membrane you may use one of the prestained protein markers. These marker proteins are stained covalently. Due to the staining the molecular weight differs from the unstained protein and is, therefore, not suitable for molecular weight determination.
SDS PAGE Protein Standards
Native PAGE Protein Standards
-
-
SERVALYT™ Carrier Ampholytes SERVALYT™ Carrier Ampholytes are low molecular weight molecules of zwitterionic character. They are a mixture of synthetically derived species of avera...
-
IPG Strips for 1D / 2D GE SERVA IPG BlueStrips are dried gel strips with immobilized pH gradient used in high resolution 2D gel electrophoresis of proteins. The strips have to be r...
-
Detection Reagents Before membrane bound antigens can be detected, unspecific binding sites for antibodies on the transfer membrane have to be blocked. For many applications skim ...
-
Agarose Media The electrophoretic behavior in gels of DNA and RNA is effected by their size and shape. Both RNA and DNA can exist as single and double stranded molecules and the s...
-
Staining Kits Depending on the required sensitivity protein gels are Coomassie®-, silver or fluorescence stained after electrophoresis.Besides SERVA DensiStain Blue G for rapi...
-
New Brochure: Instruments for Life Sciences Research! Electrophoresis Systems & Laboratory Equipment: Vertical Electrophoresis Horizontal Electrophoresis Power Supplies Spot ...
-
DOWEX® Ion Exchange Resins DOWEX® resins are made of spherical particles. The benefit for the user is a highly efficient exchange process, due to optimal flow-through kinetics bec...
